Table of Contents

Chapter 1 Basics of automated, high-accuracy mutation screening with the WAVE[registered] Nucleic Acid Fragment Analysis Systems; Chapter 2 Influence of PCR on DHPLC Polymorphism Characterization; Chapter 3 Denaturing High Performance Liquid Chromatography (DHPLC), an important mutation detection technique in mutation analysis of the breast and ovarian cancer predisposing genes BRCA1 and BRCA2; Chapter 4 Mutation detection in the tuberous sclerosis genes TSC1 and TSC2 by DHPLC; Chapter 5 Rapid analysis to detect base changes in human mitochondrialDNA by restriction enzyme analysis followed by denaturing high performance liquid chromatography; Chapter 6 Mutation analysis of LDLR mutations in patients with familial hypercholesterolaemia; Chapter 7 Novel DHPLC based strategy for identification and characterization of (bi)-clonal T-cell receptor targets for the detection of minimal residual disease in childhood acute lymphoblastic leukemia; Chapter 8 SIRPH: a rapid, quantitative SNuPE-IP RP HPLC method for simultaneous methylation analysis at multiple CpG sites; Chapter 9 Isolation and sequencing of heteroduplex fragments from DHPLC analysis of somatic, low-f requency mutations; Chapter 10 Analysis of promoter methylation using denaturing high-performance liquid chromatography (DHPLC)

Reviews

Genetic Variance Detection - Technologies for Pharmacogenomics is Karl Hecker's second book about technologies for genetic variance detection. It broadly covers a wide range of technologies currently used for genetic variance detection and constitutes an essential contribution to the field. A reference guide for every molecular biology laboratory. His first book Genetic Variance Detection - Nuts & Bolts of DHPLC in Genomics (ISBN 0966402774) provides comprehensive and in depth coverage of denaturing high-performance liquid chromatography and is an invaluable reference for anyone using or planning to use DHPLC for genetic variance detection. -- Norbert Arnold, Professor Dr. rer. nat.