Table of Contents

Preface vii


Acknowledgments ix


Authors xi


Chapter 1 Fecal Examination for the Diagnosis of Parasitism 3


Collection of Fecal Samples 3


Storage and Shipment of Fecal Samples 4


Fecal Exam Procedures 4


Fecal Flotation 4


Additional Procedures for Fecal Examination 12


Quality Control for Fecal Exam Procedures 15


Use of the Microscope 16


Microscope Calibration 16


Pseudoparasites and Spurious Parasites 19


Identification of Nematode Larvae Recovered with Fecal Flotation or Baermann Procedures 23


Techniques for Evaluation of Strongylid Nematodes in Grazing Animals 28


Fecal Culture 28


Identification of Ruminant and Camelid Third-Stage Larvae 29


Identification of Horse Third-Stage Larvae 30


Fecal Egg Count Reduction Test 30


Identification of Adult Worms 38


Hoyer’s Solution 39


Lactophenol 39


Parasites of Domestic Animals 39


Dogs and Cats 40


Ruminants and Camelids 88


Horses 114


Swine 128


Birds 140


Rodents and Rabbits 156


Reptiles 164


Chapter 2 Detection of Protozoan and Helminth Parasites in the Urinary, Reproductive, and Integumentary Systems and in the Eye 171


Techniques for Parasite Recovery 171


Parasites of the Urinary System 171


Parasites of the Reproductive Tract 171


Helminth Parasites of the Integumentary System 172


Urinary System Parasites 174


Reproductive System Parasites 178


Parasites of Other Systems (Excluding Arthropods) 178


Chapter 3 Detection of Parasites in the Blood 185


Immunologic Detection of Blood Parasites 185


Microscopic Examination of Blood for Protozoan Parasites 185 Giemsa Stain 186


Microscopic Examination of Blood for Nematode Parasites 187


Tests for Canine Heartworm Microfilariae in Blood Samples 188


Blood Parasites of Dogs and Cats 191


Blood Parasites of Livestock and Horses 202


Blood Parasites of Birds 208


Chapter 4 Immunodiagnostic and Molecular Diagnostic Tests in Veterinary Parasitology 213


Immunodiagnostic Methods 213


Molecular Diagnostic Methods 216


Chapter 5 Diagnosis of Arthropod Parasites 217


Subclass Acari (Mites and Ticks) 217


Mite Identification 217


Tick Identification 246


Class Insecta 264


Lice (Orders Anoplura and Mallophaga) 266


Fleas (Order Siphonaptera) 280


Flies (Order Diptera) 288


Other Insects 302


Chapter 6 Parasites of Fish 305


Techniques for Recovery of Ectoparasites 305


Skin Biopsy (Mucus Smear) 305


Fin Biopsy (Fin Snip) 306


Gill Biopsy (Gill Snip) 307


Recovery of Endoparasites 307


Parasites of Fish 308


Bibliography 325


Index 327

About the Author

Anne M. Zajac, DVM, PhD, is Associate Professor in theDepartment of Biomedical Sciences and Pathobiology atVirginia-Maryland Regional College of Veterinary Medicine, VirginiaTech, Blacksburg, Virginia. Gary A. Conboy, DVM, PhD, is Associate Professor in theDepartment of Pathobiology and Microbiology at Atlantic VeterinaryCollege, University of Prince Edward Island, Charlottetown, PrinceEdward Island, Canada.

Reviews

This book is one which the reviewer can see as being veryuseful both in the veterinary technology classroom as well as onthe bench of a working laboratory. (VeterinaryInformation Network, 9 January 2013) Overall, an excellent reference book with outstandingimages and aids to parasite identification. I have utterly enjoyedthe opportunity to review this book and will value its addition toour lab bookshelf . (Veterinary Record,15 June 2013) "This update would be an excellent resource for anyone involvedin veterinary parasitology, practitioners as well as students. Itprovides practical, useful, and up-to-dateinformation. (Doody s, 17 August2012)