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Analytical ultracentrifugation is a powerful and rigorous method to analyze the size, shape. and interactions of macromolecules in solution. This volume in the Methods in Enzymology series represents up-to-date knowledge and protocols.

Table of Contents

1. Next Generation AUC adds a Spectral Dimension: Development of Multiwavelength Detectors for the Analytical Ultracentrifuge
Joseph Pearson, Frank Krause, Dirk Haffke, Borries Demeler, Kristian Schilling and Helmut Cölfen
2. Next Generation AUC: Analysis of Multi-Wavelength Analytical Ultracentrifugation Data
Gary E. Gorbet, Joseph Z. Pearson, Aysha K. Demeler, Helmut Cölfen and Borries Demeler
3. Sedimentation Velocity: A Classical Perspective
John J. Correia and Walter F. Stafford
4. Hydrodynamic Modeling and its Application in AUC
Mattia Rocco and Olwyn Byron
5. Calculations and Publication-Quality Illustrations for Analytical Ultracentrifugation Data
Chad A. Brautigam
6. Sedimentation Equilibrium Analysis of Clpb Self-Association in Diluted and Crowded Solutions.
Carlos Alfonso, Urko del Castillo, Ianire Martín, Arturo Muga and Germán Rivas
7. Analysis of Linked Equilibria
JiaBei Lin and Aaron L. Lucius
8. Elucidating Complicated Assembling Systems in Biology Using Size-And-Shape Analysis of Sedimentation Velocity Data
Catherine T. Chaton and Andrew B. Herr
9. Quaternary Structure Analyses of an Essential Oligomeric Enzyme
Tatiana P. Soares da Costa, Janni B. Christensen, Sebastien Desbois, Shane E. Gordon, Ruchi Gupta, Campbell J. Hogan, Tao G. Nelson, Matthew T. Downton, Chamodi K. Gardhi, Belinda M. Abbott, John Wagner, Santosh Panjikar and Matthew A. Perugini
10. Characterization of Intrinsically Disordered Proteins by Analytical Ultracentrifugation
David J. Scott and Donald J. Winzor
11. Sedimentation Velocity Analysis of the Size Distribution of Amyloid Oligomers and Fibrils
Yee-Foong Mok, Geoffrey J. Howlett and Michael D.W. Griffin
12. AUC and Small Angle Scattering for Membrane Proteins
Aline Le Roy, Kai Wang, Béatrice Schaack, Peter Schuck, Cécile Breyton and Christine Ebel
13. Hydrodynamic Models of G-quadruplex Structures
Jonathan B. Chaires, William L. Dean, Huy Le and John O. Trent
14. Analytical Ultracentrifugation as a Tool to Study Non-specific Protein-DNA Interactions
Teng-Chieh Yang, Carlos Enrique Catalano and Nasib Karl Maluf
15. Characterization of Homogeneous, Cooperative Protein-DNA Clusters by Sedimentation Equilibrium Analytical Ultracentrifugation and Atomic Force Microscopy
Ingrid Tessmer and Michael G. Fried
16. Sedimentation Velocity Analysis of Large Oligomeric Chromatin Complexes Using Interference Detection.
Hansen and Jeffrey
17. Methods in Enzymology – Analytical Ultracentrifugation
David L Bain, Rolando W De Angelis, Keith D Connaghan, Qin Yang, Gregory D Degala and James R Lambert
18. Ultracentrifuge Methods for the Analysis of Polysaccharides, Glycoconjugates and Lignins
Stephen E. Harding, Gary G. Adams, Fahad Almutairi, Qushmua Alzahrani, Tayyibe Erten, M. Samil Kök and Richard B. Gillis
19. Analytical Ultracentrifugation and its Role in Development and Research of Therapeutical Proteins.
Jun Liu, Sandeep Yadav, James Andya, Barthélemy Demeule and Steven J. Shire
20. Guidance to Achieve Accurate Aggregate Quantitation in Biopharmaceuticals by SV-AUC
Kelly K. Arthur, Brent S. Kendrick and John P. Gabrielson
21. Protein Assembly in Serum and the Differences from Assembly in Buffer.
John J. Hill and Thomas M. Laue

About the Author

Dr. Cole received a Sc.B. in Biochemistry from Brown University. He obtained his Ph.D. in Biophysical Chemistry at UC Berkeley where he investigated the mechanism of photosynthetic oxygen evolution. He carried out postdoctoral research at Stanford University on the structure and function of metalloenzymes that interact with oxygen. In 1991, he took a position at Merck Research Laboratories in antiviral drug discovery. Dr. Cole applied analytical ultracentrifugation and related biophysical techniques to probe macromolecular interactions that regulate replication of HIV, Influenza and Hepatitis C virus. In 2001, he to return to academia and moved to the Department of Molecular and Cell Biology at the University of Connecticut, where he is professor of structural biology, biophysics and biochemistry. His research group uses biophysical approaches to characterize macromolecular interactions that regulate important biological processes. A major focus is the innate immunity pathway for defense against viral infection. In an NIH-supported research program, his group has defined the mechanism for activation of protein kinase R by RNA. He directs the National Analytical Ultracentrifugation Facility and holds a joint appointment in the department of Chemistry at the University of Connecticut. He is a member of the editorial board of Biophysical Journal. He is the author of 84 publications and reviews.

Reviews

Praise for the Series:
"Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry
"The work most often consulted in the lab." --Enzymologia
"The Methods in Enzymology series represents the gold-standard." --Neuroscience