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Arbuscular Mycorrhizal Fungi
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Table of Contents

1. Isolation and Culture of Arbuscular Mycorrhizal Fungi from Field Samples

Dora Trejo-Aguilar and Jacob Banuelos

2. The Mycorrhizal Donor Plant (MDP) In Vitro Culture System for the Efficient Colonization of Whole Plants

Ismahen Lalaymia and Stephane Declerck

3. A Whole-Plant Culture Method to Study Structural and Functional Traits of Extraradical Mycelium

Cristiana Sbrana, Alessandra Pepe, Nuria Ferrol, and Manuela Giovannetti

4. Histochemical Staining and Quantification of Arbuscular Mycorrhizal Fungal Colonization

Tania Ho-Plagaro, Maria Isabel Tamayo-Navarrete, and Jose Manuel Garcia-Garrido

5. Fluorescent Staining of Arbuscular Mycorrhizal Structures Using Wheat Germ Agglutinin (WGA) and Propidium Iodide

Gennaro Carotenuto and Andrea Genre

6. Visualization of Arbuscular Mycorrhizal Fungal Extra-Radical Hyphae and Spores Vitality and Activity

Pablo Cornejo and Humberto Aponte

7. Molecular Quantification of Arbuscular Mycorrhizal Fungal Root Colonization Martina Janouskova and Petra Caklova 8. Single-Spore-Extraction for Genetic Analyses of Arbuscular Mycorrhizal Fungi

Dirk Redecker

9. High Throughput DNA Sequence-Based Analysis of AMF Communities

Iris Marisa Maxaieie Victorino, Andrea Berruti, Alberto Orgiazzi, Samuele Voyron, Valeria Bianciotto, and Erica Lumini

10. Analysis of Arbuscular Mycorrhizal Fungal Communities by Terminal Restriction Fragment Length Polymorphism (TRFLP)

Alvaro Lopez-Garcia

11. Bioinformatic Methods for the Analysis of High-Throughput RNA Sequencing in Arbuscular Mycorrhizal Fungi

Francesco Venice, Alessandra Salvioli di Fossalunga, and Paola Bonfante

12. Arbuscular Mycorrhizal Fungal Gene Expression Analysis by Real-Time PCR

Alessandro Silvestri, Jacob Perez-Tienda, and Juan Antonio Lopez-Raez

13. Laser Microdissection as a Useful Tool to Study Gene Expression in Plant and Fungal Partners in AM Symbiosis

Raffaella Balestrini and Valentina Fiorilli

14. Detection of Arbuscular Mycorrhizal Fungal Gene Expression by In Situ Hybridization

Bettina Hause and Natalia Requena

15. Functional Analyses of Arbuscular Mycorrhizal Fungal Genes in Yeast Elisabeth Tamayo, Tamara Gomez-Gallego, and Nuria Ferrol 16. Isotope Labelling to Study Phosphorus Uptake in the Arbuscular Mycorrhizal Symbiosis

Carla Cruz-Paredes and Mayra E. Gavito

17. Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycorrhizae

Ghislaine Recorbet, Pierre-Emmanuel Courty, and Daniel Wipf

18. Host-Induced Gene Silencing of Arbuscular Mycorrhizal Fungal Genes via Agrobacterium Rhizogenes-Mediated Root Transformation in Medicago truncatula

Meike Hartmann, Stefanie Voss, and Natalia Requena

19. Application of Virus-Induced Gene Silencing to Arbuscular Mycorrhizal Fungi Tatsuhiro Ezawa, Hayato Maruyama, Yusuke Kikuchi, Kaede Yokoyama, and Chikara Masuta

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