Table of Contents

1. Cell Senescence Culturing MethodsHuaping Chen, Yuanyuan Li, and Trygve O. Tollefsbol 2. Digital Image Analysis of Cells Stained with the Senescence-Associated Beta-Galactosidase AssayLiran I. Shlush and Sara Selig 3. Analysis of Biomarkers of Caloric Restriction in Aging CellsYuanyuan Li and Trygve O. Tollefsbol 4. Cell Sorting of Young and Senescent CellsGraeme Hewitt, Thomas von Zglinicki, and Joao F. Passos 5. Studying the Replicative Life Span of Yeast Cells David A. Sinclair 6. Methods for Creating Mutations in C. elegans that Extend LifespanDayong Wang, Min Cao, Jessica Dinh, and Yuqing Dong 7. Aging Studies in Drosophila MelanogasterYaning Sun, Jason Yolitz, Cecilia Wang, Edward Spangler, Ming Zhan, and Sige Zou 8. The Use of Calorie Restriction Mimetics to Study AgingMunehiro Kitada and Daisuke Koya 9. Using Somatic-Cell Nuclear Transfer to Study AgingSatoshi Kishigami, Ah Reum Lee, and Teruhiko Wakayama 10. Induction of Cellular Senescence by Oncogenic RASRomi Gupta and Narendra Wajapeyee 11. Methods of Cellular Senescence Induction Using Oxidative StressZhe Wang, Dandan Wie, and Hengyi Xiao 12. Methods of Testing Pharmacological Drugs Effects on Aging and Life-span in Mice Vladimir N. Anisimov, Irina G. Popovich, and Mark A. Zabezhinski13. Mitochondria-targeted Anti-aging Gene Therapy with Adeno-associated Viral VectorsDejia Li and Dongsheng Duan 14. Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and RegenerationNadav Kimelman Bleich, Ilan Kallai, Dmitriy Sheyn, Wafa Tawackoli , Zulma Gazit , Gadi Pelled, and Dan Gazit15. Exometabolomic Mapping of Caenorhabditis elegans - A Tool to Non-Invasively Investigate AgingRobert J. Mishur, Jeffrey A. Butler, and Shane L. Rea 16. Detecting Polymorphisms in Human Longevity Studies: HLA typing and SNP Genotyping by Amplicon SequencingGilberto Vargas-Alarcon and Carmina Flores-Dominguez 17. Gel Electrophoresis-based Proteomics of Senescent TissuesSteven Carberry and Kay Ohlendieck 18. Single-Cell Semiconductor Sequencing Andrea B. Kohn, Tatiana P. Moroz, Jeffrey P. Barnes, Mandy Netherton, and Leonid L. Moroz 19. Application of DNA Microarray Technology to Gerontological StudiesKiyoshi Masuda, Yuki Kuwano, Kensei Nishida, and Kazuhito Rokutan

Carmen M. Koch and Wolfgang Wagner 21. Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of AgingLeonid L. Moroz and Andrea B. Kohn