Table of Contents

Cell Senescence Culturing Methods.- Digital Image Analysis of Cells Stained with the Senescence-Associated -Galactosidase Assay.- Analysis of Biomarkers of Caloric Restriction in Aging Cells.- Cell Sorting of Young and Senescent Cells.- Studying the Replicative Life Span of Yeast Cells.- Methods for Creating Mutations in C. elegans that Extend Lifespan.- Aging Studies in Drosophila Melanogaster.- The Use of Calorie Restriction Mimetics to Study Aging.- Using Somatic-Cell Nuclear Transfer to Study Aging.- Induction of Cellular Senescence by Oncogenic RAS.- Methods of Cellular Senescence Induction Using Oxidative Stress.- Methods of Testing Pharmacological Drugs Effects on Aging and Life-span in Mice.- Mitochondria-targeted Anti-aging Gene Therapy with Adeno-associated Viral Vectors.- Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration.- Exometabolomic Mapping of Caenorhabditis elegans – A Tool to Non-Invasively Investigate Aging.- Detecting Polymorphisms in Human Longevity Studies: HLA typing and SNP Genotyping by Amplicon Sequencing.- Gel Electrophoresis-based Proteomics of Senescent Tissues.- Single-Cell Semiconductor Sequencing.- Application of DNA Microarray Technology to Gerontological Studies.- Epigenetic Biomarker to Determine Replicative Senescence of Cultured Cells.- Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of Aging.