Table of Contents

1. Bioluminescence Imaging: Basics and Practical Limitations Christian E. Badr Part I: In Vitro Bioassays 2. Extraction and Quantification of Adenosine Triphosphate in Mammalian Tissues and Cells Junji Chida and Hiroshi Kido 3. Neuronal Network Imaging in Acute Slices Using Ca2+ Sensitive Bioluminescent Reporter Ludovic Tricoire and Bertrand Lambolez 4. Gaussia Luciferase-Based Mycoplasma Detection Assay in Mammalian Cell Culture M. Hannah Degeling, M. Sarah S. Bovenberg, Marie Tannous, and Bakhos A. Tannous Part II: Imaging Molecular and Cellular Events 5. Split Gaussia Luciferase for Imaging Ligand-Receptor Binding Kathryn E. Luker and Gary D. Luker 6. Video-Rate Bioluminescence Imaging of Protein Secretion from a Living Cell Takahiro Suzuki and Satoshi Inouye 7. Bioluminescence Reporter Gene-Based Detection of MicroRNAs Hae Young Ko, Young Sik Lee, and Soonhag Kim 8. Monitoring of Transcriptional Dynamics of HIF and NF B Activities Miguel A.S. Cavadas and Alex Cheong 9. Real-Time Bioluminescent Tracking of Cellular Population Dynamics Dan Close, Tingting Xu, Steven Ripp, and Gary Sayler 10. Fabrication of Bioluminescent Capsules and Live-Cell Imaging Sung Bae Kim Part III: Imaging of Disease (Pathogenesis) 11. The Bioluminescent Imaging of Spontaneously Occurring Tumors in Immunocompetent ODD-Luciferase Bearing Transgenic Mice Scott J. Goldman and Shengkan Jin 12. Blood-Based Assay with Secreted Gaussia Luciferase to Monitor Tumor Metastasis Hiroshi Yamashita, Dan T. Nguyen, and Euiheon Chung 13. Bioluminescence Imaging of Fungal Biofilm Development in Live Animals Greetje Vande Velde, Sona Kucharikova, Patrick Van Dijck, and Uwe Himmelreich 14. Bioluminescent Imaging of Bacteria During Mouse Infection Jonathan M. Warawa and Matthew B. Lawrenz Part IV: Therapeutic Imaging 15. Cell-Based Bioluminescence Screening Assays Romain J. Amante and Christian E. Badr 16. Bioluminescence-Based Monitoring of Virus Vector-Mediated Gene Transfer in Mice Casey A. Maguire17. Simultaneous In Vivo Monitoring of Regulatory and Effector T Lymphocytes Using Secreted Gaussia Luciferase, Firefly Luciferase, and Secreted Alkaline Phosphatase Grant K. Lewandrowski, Ciara N. Magee, Marwan Mounayar, Bakhos A. Tannous, and Jamil Azzi Part V: Emerging Technologies18. Multiplex Functional Bioluminescent Reporters Using Gaussia Luciferase Fused to Epitope Tags in an Immunobinding Assay Sjoerd van Rijn, Thomas Wurdinger, and Jonas Nilsson19. Non-Invasive In Vivo Monitoring of Extracellular Vesicles Charles P. Lai, Bakhos A. Tannous, and Xandra O. Breakefield20. In Vitro and In Vivo Demonstrations of Fluorescence by Unbound Excitation from Luminescence (FUEL) Joe Dragavon, Abdessalem Rekiki, Ioanna Theodorou, Chelsea Samson, Samantha Blazquez, Kelly L. Rogers, Regis Tournebize, and Spencer Shorte

Reviews

From the reviews:

"The intent is to provide diverse group of techniques to everyone interested in implementing bioluminescence-based imaging regardless of their level of experience with such methodologies. These are very worthy objectives and the book successfully meets them. ... The book is illustrated with high-quality pictures and figures. This is an excellent source of protocols and methodologies that use bioluminescence imaging as a tool for visualizing and tracking various biological processes in diverse fields." (Omer Iqbal, Doody's Book Reviews, January, 2014)