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Biomolecular Imaging at High Spatial and Temporal Resolution In Vitro and In Vivo


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Table of Contents

Introduction.- Biomolecules & Microscopy.- Materials and Methods.-Experimental Methods.- Imaging Methods.- Experimental Results.- Towards an Atomistic Structure of a De Novo Designed Peptide Fibre.- Developing Novel Probes for Correlative Light Electron Microscopy.- Conclusions and Appendices.- Concluding Remarks.- Abbreviations.- Amino Acid Structures.- Vectors, Primers and Recipes.

About the Author

Thom Sharp completed his bachelor's degree in Biochemistry at the University of Bristol, with a year spent a year working at AstraZeneca. He continued in Bristol for his PhD in bionanotechnology, which was performed under the supervision of Dek Woolfson, a protein design chemist, and Paul Verkade, an electron microscopist. Thom is currently at the University of Oxford, where he is developing DNA-templated protein arrays to increase the throughput of single-particle analysis.

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