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Updated classic and highly innovative techniques essential to the study of Caenorhabditis Elegans

Table of Contents

Culturing methods and genetic proceduresGeneral methods of culture: Jim LewisMutagenesis: Phil AndersonGenetic screening methods: James Thomas, Eric Jorgensen, Eric LambieGenetic mapping methods: Ben Williams, Stephen Wicks (Boston College)Genetic mosaic analysis: Robert HermanUse of specialized chromosomes and rearrangements: Ann Rose, David BaillieGene knockouts: Shohei Mitani, Robert BarsteadRNAi and RNAi screening methods: Julie Ahringer, Andrew Dillin

Genomics and BioinformaticsTranscriptional profiling: Stuart Kim, Don Moerman, Valerie ReinkeMicroRNA methods: Victor Ambros, Frank Slack, Amy PasquinelliAnalysis of RNA function: Marv Wickens, Judith KimbleAnalysis of the interactome: Marc VidalWormBase: Eric Schwartz, Paul Sternberg, Lincoln SteinStructural genomics: Ming Luo, Larry DeLucas, Songlin Li, Mike Carson (U. Alabama)Proteomics: Ahna Skop, Moerman D., Isobe T, Krijgsveld, JTranscriptional regulatory networks: Marion Walhout

Markers for gene expression and protein localizationImmunofluorescence and antibodies: David MillerImaging: John White, William MohlerElectron Microscopy: David HallProteins and protein assemblies: Henry EpsteinAnalysis of morphogenesis: Jeff Hardin, Andrew ChisholmCell death: Monica Driscoll, Ding Xue