Table of Contents

Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction.- Imaging Cancer Metabolism with Positron Emission Tomography (PET).- Radioluminescence microscopy - A Quantitative Method for Radioisotopic Imaging of Metabolic Fluxes in Living Cancer Cells.- Use of 13C315N1-Serine or 13C515N1-Methionine for Studying Methylation dynamics in Cancer Cell Metabolism and Epigenetics.- Measurement of Mitochondrial Membrane Potential with the Fluorescent Dye Tetramethylrhodamine Methyl Ester (TMRM).- Assessment of Stabilization and Activity of the HIFs Important for Hypoxia-induced Signalling in Cancer Cells.- Determination of Polarization of Resident Macrophages and their Effect on the Tumor Microenvironment.- Quantitation of Macropinocytosis in Cancer Cells.- Integrated Analysis of Acetyl-CoA and Histone Modification via Mass Spectrometry to Investigate MetabolicallyDriven Acetylation.- Methods to Measure Autophagy in Cancer Metabolism.- Lipidomic Analysis of Cancer Cell and Tumor Tissue.- Mass Spectrometry-based Profiling of Metabolites in Human Biofluids.- Gas Chromatography-Mass Spectrometry and Analysis of the Serum Metabolomic Profile through Extraction and Derivatization of Polar Metabolites.- Metabolite Profiling of Clinical Cancer Biofluid Samples by NMR Spectroscopy.- Assessment of Metabolic Signature for Cancer Diagnosis Using Desorption Electrospray Ionization Mass Spectrometric Imaging.- Compositional Analysis of the Human Microbiome in Cancer Research.- Assessing Metabolic Dysregulation in Muscle During Cachexia.- Using Seahorse Machine to Measure OCR and ECAR in Cancer Cells.- Metabolic Profiling of Live Cancer Tissues using NAD(P)H fluorescence Lifetime Imaging.- Overview of Characterizing Cancer Glycans with Lectin-based Analytical Methods.- Hyperpolarized MRI for Studying Tumor Metabolism.- Overview of Glutamine-Dependency and Metabolic Rescue Protocols.- Integration of Metabolomics and Transcriptomics to Identify Gene-metabolite Relationships Specific to Phenotype.- Biclustering Analysis of Co-regulation Patterns in Nuclear Encoded Mitochondrial Genes and Metabolic Pathways.- Using the Human Genome-scale Metabolic Model Recon 2 for Steady-state Flux Analysis of Cancer Cell Metabolism.