Table of Contents

1. Production of Eukaryotic Cell-Free Lysate from Leishmania tarentolaeWayne Johnston and Kirill Alexandrov 2. Bioinformatics Analysis and Optimization of Cell-Free Protein ExpressionAlexander A. Tokmakova, Atsushi Kurotania, Mikako Shirouzu, Yasuo Fukamid, and Shigeyuki Yokoyama 3. A Cell-Free Expression Screen to Identify Fusion Tags for Improved Protein ExpressionAndrew Kralicek 4. One-Pot, Microscale Cell-Free Enzyme Expression and ScreeningAarthi Chandrasekaran and Anup K. Singh 5. Cell-Free Translation of Biofuels EnzymesTaichi E. Takasuka, Johnnie A. Walker, Lai F. Bergeman, Kirk A. Vander Meulen, Shin-ichi Makino, Nathaniel L. Elsen, and Brian G. Fox 6. Cloning-Independent Expression and Screening of Enzymes using Cell-Free Protein Synthesis SystemsYong-Chan Kwon, Jae-Kwang Song, and Dong-Myung Kim 7. High Level Cell-Free Production of Membrane Proteins with NanodiscsChristian Roos, Lei Kai, Stefan Haberstock, Davide Proverbio, Umesh Ghoshdastider, Yi Ma, Slawomir Filipek, Xiaoning Wang, Volker Doetsch, and Frank Bernhard 8. Cell Free Protein Based Enzyme Discovery and Protein-Ligand Interaction StudySabrina Guillemer, Cecile Persillon, Jean-Michel Masson, and Gilles Ravot 9. Human Cell Extract-Derived Cell-Free Systems for Virus SynthesisTominari Kobayashi, Kodai Machida, and Hiroaki Imataka 10. Cell-Free Protein Synthesis in Microfluidic 96-Well PlatesKirsten Jackson, Ruba Khnouf, and Z. Hugh Fan 11. Preparation of Multiple Site-Specific Mutant Proteins for NMR Studies by PCR-Directed Cell-Free Protein SynthesisKiyoshi Ozawa and Ruhu Qi 12. Site-Specific Incorporation of Unnatural Amino Acids into Proteins by Cell-Free Protein SynthesisKiyoshi Ozawa and Choy Theng Loh 13. In vitro Translation of Papillomavirus Authentic and Codon-Modified L1 Capsid Gene mRNAs in Mouse Keratinocyte Cell-Free LysateKong-Nan Zhao 14. An Optimized Yeast Cell-Free Lysate System for In vitro Translation of Human Virus mRNAXiao Wang, Liang Zhao, and Kong-Nan Zhao 15. In vitro Translation Based Protein Kinase Substrate IdentificationSzilvia K. Nagy and Tamas Meszaros 16. Preparation of Protein Arrays using Cell-Free Protein ExpressionElizabeth A. Cook and Mingyue He 17. Post-Transcriptional Control of Protein Synthesis in Drosophila S2 Cell-Free SystemMotoaki Wakiyama and Shigeyuki Yokoyama 18. Cell-Free Membrane Protein Expression Tomomi Kimura-Soyema, Mikako Shirouzu, and Shigeyuki Yokoyama 19. The PURE System for Protein ProductionYoshihiro Shimizu, Yutetsu Kuruma, Takashi Kanamori, and Takuya Ueda 20. A Cell-Free Protein Synthesis System from Insect CellsToru Ezure, Takashi Suzuki, and Eiji Ando 21. A Cell-Free Expression Platform for Production of Protein MicroarraysXristo Zarate and David W. Galbraith