Table of Contents

1. An introduction to cell imaging (David Stephens)
2. Confocal or wide-field? A guide to selecting appropriate methods for cell imaging (Mark A. Jepson)
3. Image quantification and analysis (Kota Miura and Jens Rietdorf)
4. Transmitted light imaging (Jon D. Lane and Howard Stebbing)
5. Spectral imaging techniques for fluorescence microscopy (Timo Zimmermann)
6. Measurement of protein motion by photobleaching (John F. Presley)
7. Imaging calcium and calcium-binding proteins ( Burcu Hasdemir, Robert Burgoyne and Alexei Tepikin)
8. Total internal reflection fluorescence microscopy (David Zenisek)
9. An overview of three-dimensional and four-dimensional microscopy by computational deconvolution (Jose-Angel Conchello)
10. Correlative microscopy using Tokuyasu cryosections: applications for immunolabeling and in situ hybridization (Miguel R. Branco, Sheila Q. Xie, et al.)
11. Fluorescence lifetime imaging (Klaus Suhling)
12. Homo-FRET measurements to investigate molecular-scale organization of proteins in living cells (Rajat Varma and Satyajit Mayor)
13. High-content and high-throughput screening (Elizabeth P. Roquemore)
List of suppliers
Index

Reviews

"It is clear that experienced practitioners have written this book. The major strength of the book lies in the protocols at the end of each chapter. It is true that 'there is no substitute for experience' but a lot of distilled wisdom is clearly presented throughout for the immediate benefit of beginner or experienced microscopists alike. I wholeheartedly recommend it, advise you to buy it and expect my copy to become a well-thumbed friend" RMS Proceedings, March 2006