Table of Contents

Preface
David Caballero, Subhas C Kundu and Rui L Reis

Section 1: Decellularization and characterization methods

1.A step-by-step protocol for generating human fibroblast cell-derived completely biological extracellular matrix scaffolds
Dhavan Sharma, Morgan Ferguson and Feng Zhao

2.Preparation of osteogenic matrices from cultured cells
Carl A. Gregory, Eoin McNeill and Simin Pan

3.Decellularized hASCs-derived matrices as biomaterials for 3D in vitro approaches
Fátima Raquel Maia, Rui Luís Reis and Joaquim Miguel Oiveira

4.Characterization of the elastic properties of extracellular matrix models by atomic force microscopy
J. Otero, D. Navajas and J. Alcaraz

5.Glycosaminoglycan disaccharide compositional analysis of cell-derived extracellular matrices using liquid chromatography-tandem mass spectrometry
João C. Silva, Marta S. Carvalho, Ke Xia, Joaquim M. S. Cabral, Claúdia L. da Silva, Frederico C. Ferreira, Deepak Vashishth and Robert J. Linhardt

Section 2: Engineering cell-derived matrices with advanced characteristics

6.Engineering clinically-relevant human fibroblastic cell-derived extracellular matrices
Janusz Franco-Barraza, Kristopher S. Raghavan, Tiffany Luong and Edna Cukierman

7.Engineering cell-derived matrices with controlled 3D architectures for pathophysiological studies
Enrico Almici, David Caballero, Joan Montero Boronat and Josep Samitier Martí

8.Generation of fluorescent cell-derived-matrix to study 3D cell migration
Amélie Luise Godeau, Hélène Delanoë-Ayari and Daniel Riveline

9.Re-engineered cell-derived extracellular matrix as a new approach to clarify the role of native ECM
Insung Yong, Seung Won Oh and Pilnam Kim

Section 3: Cell-derived matrices for biomedical applications

10.Cell-derived matrices (CDM)—Methods, challenges and applications
Cheng HW, Yuan MT, Li CW and Chan BP.

11.Boosting collagen deposition with a lysyl oxidase/bone morphogenetic protein-1 cocktail
Tamara Rosell-García and Fernando Rodríguez-Pascual

12.Generating specific cell-derived matrices from human trabecular meshwork cell cultures for mechanistic studies
Felix Yemanyi, Janice Vranka and VijayKrishna Raghunathan

13.Methods in cell biology: Cell-derived matrices
Elizabeth Pavez Loriè and Petra Boukamp

About the Author

Dr. David Caballero is a senior Research Associate at the 3B’s Research Group, I3Bs – Institute for Biomaterials, Biodegradables and Biomimetics at University of Minho (Portugal). Dr. Caballero has broad experience in the fields of biophysics, bioengineering, microfluidics, and nanotechnology. His main research interests include the development of novel bioengineered in vitro models to study the mechanistic determinants of cancer cell invasion. Currently, Dr. Caballero work focuses in the development of innovative multi-organ-on-chip tumor models using engineered native-like 3D biomaterials for drug screening applications. Subhas C. Kundu, PhD, is a Research Coordinator and former European Research Area Chair and Professor at the 3B´s Research Group, I3Bs – Institute on Biomaterials, Biodegradables and Biomimetics of the University of Minho, Portugal. His research interests include silk biomaterial matrices for biomedical applications, including 3D in vitro cancer models for investigating tumour growth and progression. In addition, he is using natural-based biomaterials for 3D cancer modelling and drug screening. Dr. Rui Reis is Vice-President for Research and Innovation of University of Minho, Portugal, Director of 3B’s Research Group and Director of ICVS/3B´s Associate Laboratory, both of UMinho. He’s the CEO of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, the Coordinator of the Discoveries Centre for Regenerative and Precision Medicine, the Global Past-President of the Tissue Engineering and Regenerative Medicine International Society and the Editor-in-chief of the Journal of Tissue Engineering and Regenerative Medicine. He’s edited 18 books, 10 special issue journals, 280 book chapters and has more than 1225 published works listed on ISI Web of Knowledge, being an inventor of around 70 patents. He’s been awarded many important international prizes, including the TERMIS-EU contributions to the literature Award and the TERMIS-EU Career Achievement Award, and recently the UNESCO- International Life Sciences Award and the IET A. F. Harvey Engineering Research Prize.