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Chemical Biology


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Part I In Vitro Chemical Genetic Screening1. Identification of Therapeutic Small Molecule Leads in Cultured Cells Using Multiplexed Pathway Reporter Read-outsOzlem Kulak, Kiyoshi Yamaguchi, and Lawrence Lum2. Applying the Logic of Genetic Interaction to Discover Small Molecules That Functionally Interact with Human Disease AllelesAri D. Brettman, Pauline H. Tan, Khoa Tran, and Stanley Y. Shaw3. Construction and Application of a Photo-cross-linked Chemical ArrayYasumitsu Kondoh, Kaori Honda, and Hiroyuki Osada4. High Content Screening for Modulators of Cardiac Differentiation in Human Pluripotent Stem CellsSean Spiering, Herman Davidovics, Paul J. Bushway, Mark Mercola, and Erik Willems5. Small Molecule High-Throughput Screening Utilizing Xenopus Egg Extract Matthew R. Broadus, P. Renee Yew, Stephen R. Hann, and Ethan Lee Part II In Vivo Chemical Genetic Screening6. Fission Yeast-based High-throughput Screens for PKA Pathway Inhibitors and ActivatorsAna Santos de Medeiros, Grace Kwak, Jordan Vanderhooft, Sam Rivera, Rachel Gottlieb, and Charles S. Hoffman7. A Method for High-throughput Analysis of Chronological Aging in Schizosaccharomyces pombeJessica Stephan and Ann E. Ehrenhofer-Murray8. Protocols for the Routine Screening of Drug Sensitivity in the Human Parasite Trichomonas vaginalisManal J. Natto, Anthonius A. Eze, and Harry P. de Koning9. Chemical Genetic Screens Using Arabidopsis thaliana Seedlings Grown on Solid MediumThanh Theresa Dinh and Xuemei Chen10. Small Molecule Screening Using Drosophila Models of Human Neurological DisordersMickael Poidevin, Feiran Zhang, and Peng Jin11. High-throughput Small Molecule Screening in Caenorhabditis elegansSunitha Rangaraju, Gregory M. Solis, and Michael Petrascheck12. Whole-organism Screening for Modulators of Fasting Metabolism Using Transgenic ZebrafishPhilipp Gut and Didier Y.R. Stainier

13. High Content Screening for Modulators of Cardiovascular or Global Developmental Pathways in ZebrafishCharles H. Williams III and Charles C. Hong Part III Small Molecules in Chemical Biology14. Extraction Methods of Natural Products from Traditional Chinese MedicinesJingyu Zhang, Jianying Han, Ayokunmi Oyeleye, Miaomiao Liu, Xueting Liu, and Lixin Zhang15. Bioassay-guided Identification of Bioactive Molecules from Traditional Chinese MedicinesJianying Han, Jingyu Zhang, Wenni He, Pei Huang, Ayokunmi Oyeleye, Xueting Liu, and Lixin Zhang16. NMR Screening in Fragment-Based Drug Design: A Practical GuideHai-Young Kim and Daniel F. Wyss 17. Practical Strategies for Small Molecule Probe Development in Chemical BiologyJonathan E. Hempel and Charles C. Hong18. Principal Component Analysis as a Tool for Library Design: A Case Study Investigating Natural Products, Brand-name Drugs, Natural Product-like Libraries, and Drug-like LibrariesTodd A. Wenderski, Christopher F. Stratton, Renato A. Bauer, Felix Kopp, and Derek S. Tan19. Small Molecule Library Screening by Docking with PyRxSargis Dallakyan and Arthur J. Olson Part IV Small Molecule Target Identification20. Fluorous Photoaffinity Labeling to Probe Protein-Small Molecule InteractionsWeigang Huang and Qisheng Zhang21. Identification of the Targets of Biologically Active Small Molecules Using Quantitative ProteomicsGloria Vendrell-Navarro, Andreas Brockmeyer, Herbert Waldmann, Petra Janning, and Slava Ziegler22. Drug Affinity Responsive Target Stability (DARTS) for Small Molecule Target IdentificationMelody Y. Pai, Brett Lomenick, Heejun Hwang, Robert Schiestl, William McBride, Joseph A. Loo, and Jing Huang

23. Chemical Genomic Profiling via Barcode Sequencing to Predict Compound Mode of Action

Jeff S. Piotrowski, Scott W. Simpkins, Sheena C. Li, Raamesh Deshpande, Sean J. McIlwain, Irene M. Ong, Chad L. Myers, Charlie Boone, and Raymond J. Andersen24. Image-Based Prediction of Drug Target in YeastShinsuke Ohnuki, Hiroki Okada, and Yoshikazu Ohya

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