Table of Contents

1. Advances in Confocal Microscopy and Selected Applications

W. Matt Reilly and Christopher J. Obara

2. Choosing Fluorescent Probes and Labeling Systems

Jacoby Morris and George H. Patterson

3. General Considerations for Acquiring a Three-color Image by Laser Scanning Confocal Microscopy

Joseph A. Brzostowski

4. Microfabricated devices for confocal microscopy on biological samples

Nicole Y. Morgan

5. Zeiss Airyscan: Optimizing Usage for Fast, Gentle, Super-resolution Imaging

Xufeng Wu and John A. Hammer

6. High-resolution multicolor imaging of mitochondria in lymphocytes

Munir Akkaya, Pietro Miozzo, and Margery G. Smelkinson

7. Protein-Retention Expansion Microscopy (ExM): Scalable and Convenient Super-Resolution Microscopy

Paul Tillberg

8. Analysis of B Cell Receptor-Mediated Antigen Extraction by B lymphocytes from Plasma Membrane Sheets Using Confocal Microscopy

Abhijit Ashok Ambegaonkar and Haewon Sohn

9. Analysis of Intracellular Vesicles in B Lymphocytes: Antigen Traffic in the Spotlight

Sara Hernandez-Perez, Marika Vainio, Vid Sustar, and Pieta K. Mattila

10. Visualizing Key Signaling Components of Macropinocytosis and Phagocytosis Using Confocal Microscopy in the Model Organism Dictyostelium discoideum

Xuehua Xu, Joseph Brzostowski, Sharmila Ramachandra, Smit Bhimani, Yan You, and Tian Jin

11. Imaging GPCR-mediated Signal Events Leading to Chemotaxis and Phagocytosis

Miao Pan and Tian Jin

12. High-throughput Imaging of Arrays of Fluorescently Tagged Yeast Mutant Strains

Mojca Mattiazzi Usaj, Dara S Lo, Ben T Grys, and Brenda J Andrews

13. Visualizing the Dynamics of T Cell-Dendritic Cell Interactions in Intact Lymph Nodes By Multiphoton Confocal Microscopy

Billur Akkaya, Olena Kamenyeva, Juraj Kabat, and Ryan Kissinger

14. Studying Neuronal Biology Using Spinning Disc Confocal Microscopy

Javier Manzella-Lapeira, Joseph Brzostowski, and Jenny Serra-Vinardell

15. Method for Acute Intravital Imaging of the Large Intestine in Live Mice

Erreni Marco, Doni Andrea and Roberto Weigert

16. Fluorescence Lifetime Imaging as a Non-invasive Tool to Study Plasmodium Falciparum Metabolism

Javier Manzella-Lapeira and Joseph Brzostowski

17. Developing Analysis Protocols for Monitoring Intracellular Oxygenation using Fluorescence Lifetime Imaging of Myoglobin-mCherry

Greg Alspaugh, Branden Roarke, Alexandra Chand, Rozhin Penjweini, Alessio Andreoni, and Jay R. Knutson

18. FLIM Imaging for Metabolic Studies in Live Cells

Heejun Choi

19. A Step by Step Guide to Instant Structured Illumination Microscopy (ISIM)

Alexander Zhovmer and Christian A. Combs