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Crystals, X-Rays and Proteins


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Table of Contents

Part I: Fundamentals 1: The crystalline state and its study 2: Vector analysis and complex algebra 3: Crystal systematics 4: Waves and electromagnetic radiation 5: Fourier transforms and convolutions 6: Diffraction Part II: Diffraction Theory 7: Diffraction by one-dimensional obstacles 8: Diffraction by a three-dimensional lattice 9: The contents of the unit cell Part III: Structure Solution 10: Experimental techniques: sample preparation 11: Experimental techniques: data collection and analysis 12: The phase problem and the Patterson function 13: Molecular replacement 14: Solving the phase problem experimentally 15: Model-building and refinement 16: Complementary diffraction methods

About the Author

Dennis Sherwood read Natural Sciences as a scholar at Clare College, Cambridge, and subsequently won a Mellon Fellowship to the Department of Molecular Biophysics and Biochemistry at Yale University (MPhil), and a Calbiochem Scholarship to the University of California at San Diego (PhD). After a brief period as an ICI Post-doctoral Fellow at the University of Sussex, Dennis changed career, and joined Deloitte Haskins & Sells as a trainee consultant, and where, for 12 years, he was a consulting partner. Dennis was subsequently an Executive Director with Goldman Sachs, a partner in Bossard Consultants, and Managing Director in the UK of SRI Consulting. Dennis now runs his own business, The Silver Bullet Machine Manufacturing Company Limited , which specialises in organizational creativity and innovation. Dennis participates in a number of academic programmes at institutions such as London Business School, the London School of Economics, the University of St Gallen, and London South Bank University. Jon Cooper is a Professor of Structural Biology at UCL Department of Medicine who specialises in expression and X-ray structure analysis of proteins. Previously he was based in the School of Biological Sciences at the University of Southampton where he taught biochemistry and structural biology on undergraduate programmes and at the post-graduate level. He has been working in the protein crystallography field since the mid-1980s when he started a PhD at Birkbeck College London where he later became a post-doctoral fellow and subsequently a lecturer. He is a member of Biological Structures Group of the British Crystallographic Association (BCA) and has been a tutor at the BCA Protein Crystallography Summer School.


`The first two-thirds of this book was like a thriller to me. Even though I knew the answer, I wanted to see how the author would address the next topic and I could not put it down.' Joseph D. Ferrara, Ph.D, Crystallography Times `This is one of the best crystallography books ever written, and it is with pleasure that I wholeheartedly recommend it.' Nicholas M. Glykos, Democritus University of Thrace, Greece `The authors have nicely brought the bibliography up to date and mention recent method developments, giving a good first grasp of what is involved in solving a structure. The text also makes good use of accompanying, illustrative figures, which is most essential when developing the complex concepts of diffraction, Fourier transformation and convolution.' E. von Castelmur and A. Perrakis, Crystallography Reviews `In my opinion, this book would be the perfect textbook for a theoretical course on macromolecular crystallography' Manfred S. Weiss, Acta Crystallographica Section D `A welcome addition to any structural biology laboratory, [and] an invaluable reference, answering questions in an accurate and transparent manner' Karen McLuskey, Chemistry World

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