Table of Contents

In Vitro Protocols for Studying Expression and Activity of Cyclooxygenase Enzymes and Characterisation of Their Inhibition Patterns.- In Vitro Cyclo-oxygenase Expression and Activity Protocols: Introduction to Part I.- Techniques Used to Study Regulation of Cyclooxygenase-2 Promoter Sites.- Purification of Recombinant Human COX-1 and COX-2.- Cloning and Expression of Cyclooxygenase-1 and Cyclooxygenase-2.- Expression of Cyclooxygenase Isoforms in the Baculovirus Expression System.- Peroxidase Active Site Activity Assay.- Study of Inhibitors of the PGH Synthases for which Potency Is Regulated by the Redox State of the Enzymes.- Different Methods for Testing Potential Cyclooxygenase-1 and Cyclooxygenase-2 Inhibitors.- In Vitro Cyclooxygenase Activity Assay in Tissue Homogenates.- Techniques Used to Characterize the Binding of Cyclooxygenase Inhibitors to the Cyclooxygenase Active Site.- In Vitro Assays for Cyclooxygenase Activity and Inhibitor Characterization.- Extraction and Measurement of Prostanoids and Isoprostanes.- Extraction and Measurement of Prostanoids and Isoprostanes: Introduction to Part II.- Prostanoid Extraction and Measurement.- Measurement of 8-Iso-Prostaglandin F2? in Biological Fluids as a Measure of Lipid Peroxidation.- In Vivo Models to Study Involvement of Cyclooxygenase Products in Health and Disease.- In Vivo Models to Study Cyclooxygenase Products in Health and Disease: Introduction to Part III.- Protocols to Assess the Gastrointestinal Side Effects Resulting from Inhibition of Cyclo-Oxygenase Isoforms.- Cyclooxygenase Enzymes and Their Products in the Carrageenan-Induced Pleurisy in Rats.- Iloprost-Induced Nociception: Determination of the Site of Anti-nociceptive Action of Cyclooxygenase Inhibitors and the Involvement of CyclooxygenaseProducts in Central Mechanisms of Nociception.