Table of Contents

The Amoebozoa.- The Model Organism Dictyostelium discoideum.-Comparative Genomics of the Dictyostelids.- One Stop Shop for Everything Dictyostelium: DictyBase and the Dicty Stock Center in 2012.- Fluorescent Reporters and Methods to Analyze Fluorescent Signals.-Collection and Cultivation of Dictyostelids from the Wild.-Identification and Verification of microRNAs by High-throughput Sequencing.- Transcriptional Profiling of Dictyostelium with RNA Sequencing.- Analysis of Chromatin Organization by Deep Sequencing Technologies.- Pharmacogenetics of Resistance to Cisplatin and Other Anti-cancer Drugs and the Role of Sphingolipid Metabolism.-N-glycomic and –Glycoproteomic Studies in the Social Amoebae.-Measuring Cheating, Fitness, and Segregation in D. discoideum.-The Application of the Cre-loxP System for Generating Multiple Knock-Out and Knock-In Targeted Loci.- Extrachromosomal Inducible Expression.- Isolation of Dictyostelium Nuclei for Light and Electron Microscopy.- Investigation of DNA Repair Pathway Activity.- Transcript Localization in D. discoideum Cells by RNA FISH.- Analysis of Mitochondrial Gene Expression.- Mitochondrial Respiratory Complex Function and the Phenotypic Consequences of Dysfunction.- Micropipette Aspiration for Studying Cellular Mechanosensory Responses and Mechanics .- Quantitative Analysis of Phagocytosis and Phagosome Maturation.- Setting Up and Monitoring an Infection of Dictyostelium discoideum with Mycobacteria.- Isolation of Pathogen-containing Vacuoles.- Immuno-magnetic Purification of Fluorescent Legionella-containing Vacuoles.- Secretory Lysosomes in Dictyostelium: Visualization, Characterization and Dynamics.-  Monitoring Autophagy in Dictyostelium.