Table of Contents

List of Contributors x

Preface xvii

About the Editor xix

Acknowledgments xxi

1 An Overview of Genome-Engineering Methods 1
Sushmita, Gurminder Kaur, Santosh Kumar Upadhyay, and Praveen Chandra Verma

2 Distribution of Nutritional and Mineral Components in Important Crop Plants 22
Katarina Vogel-Mikuš, Paula Pongrac, Ivan Kreft, Primož Pelicon, Primož Vavpetič, Boštjan Jenčič, Johannes Teun van Elteren, Peter Kump, Sudhir P. Singh, and Marjana Regvar

3 Application of Genome Engineering Methods for Quality Improvement in Important Crops 43
Sajid Fiaz, Sher Aslam Khan, Galal Bakr Anis, Habib Ali, Mohsin Ali, Kazim Ali, Mehmood Ali Noor, Sibtain Ahmad, and Bilal Ahmad Asad

4 Genome Engineering for Enriching Fe and Zn in Rice Grain and Increasing Micronutrient Bioavailability 69
Conrado Dueñas, Jr., Gela Myan B. Mota, Norman Oliva, and Inez H. Slamet-Loedin

5 Development of Carotenoids Rich Grains by Genome Engineering 83
Vikrant Gautam, Gurwinder Singh Rana, Pankaj Kumar, and Santosh Watpade

6 CRISPR-Cas9 System for Agriculture Crop Improvement 97
Ashish Sharma, Poorwa Kamal Badola, and Prabodh Kumar Trivedi

7 Contribution of Crop Biofortification in Mitigating Vitamin Deficiency Globally 112
Siddhant Chaturvedi, Roni Chaudhary, and Siddharth Tiwari

8 Genome Editing Approaches for Trait Improvement in the Hairy Root Cultures of the Economically Important Plants 131
Pravin Prakash, Rakesh Srivastava, Ajay Kumar, Gurminder Kaur, and Praveen Chandra Verma

9 Phytic Acid Reduction in Cereal Grains by Genome Engineering: Potential Targets to Achieve Low Phytate Wheat 146
Ajay K. Pandey, Sipla Aggarwal, Varsha Meena, and Anil Kumar

10 Genome Engineering for Nutritional Improvement in Pulses 157
Chirag Uppal, Ajinder Kaur, and Chhaya Sharma

11 The Survey of Genetic Engineering Approaches for Oil/Fatty Acid Content Improvement in Oilseed Crops 181
Kaushal Kumar Bhati, Riyazuddin Riyazuddin, Ashish Kumar Pathak, and Anuradha Singh

12 Genome-Editing Mediated Improvement of Biotic Tolerance in Crop Plants 199
Krishan Mohan Rai and Harpal Singh

13 Genome Engineering and Essential Mineral Enrichment of Crops 210
Erum Shoeb, Uzma Badar, Srividhya Venkataraman, Ghyda Murad Hashim, and Kathleen Hefferon

14 Genome Editing to Develop Disease Resistance in Crops 224
Kashaf Zafar, Azka Noureen, Muhammad Jawad Akbar Awan, Naveed Anjum, Muhammad Qasim Aslam, Muhammad Zuhaib Khan, Imran Amin, and Shahid Mansoor

15 Biotechnological Approaches for Nutritional Improvement in Potato (Solanum tuberosum L.) 253
Chandrama Prakash Upadhyaya and Deepak Singh Bagri

16 Genome Engineering Strategies for Quality Improvement in Tomato 281
Tian Wang, Hongyan Zhang, and Hongliang Zhu

17 Genome Editing for Biofortification of Rice: Current Implications and Future Aspects 297
Suchismita Roy and Praveen Soni

18 Genome Editing for Improving Abiotic Stress Tolerance in Rice 314
Shweta Roy, Nirbhay Kumar Kushwaha, Hasthi Ram, and Praveen Soni

19 Role of Genome Engineering for the Development of Resistant Starch-Rich, Allergen-Free and Processing Quality Improved Cereal Crops 333
Anuradha Singh, Amit Yadav, Joy K. Roy, and Kaushal Kumar Bhati

20 Engineering of Plant Metabolic Pathway for Nutritional Improvement: Recent Advances and Challenges 351
Sameer Dixit, Akanchha Shukla, Vinayak Singh, and Santosh Kumar Upadhyay

21 Genome Engineering for Food Security 380
Sajid Fiaz, Sher Aslam Khan, Mehmood Ali Noor, Afifa Younas, Habib Ali, Kazim Ali, Mahmoud Mohamed Gaballah, and Galal Bakr Anis

Index 391

About the Author

SANTOSH KUMAR UPADHYAY is an Assistant Professor at the Department of Botany, Panjab University, Chandigarh, India. His present research focuses in the area of functional genomics, particularly the characterization of defense and development related genes in bread wheat.