Table of Contents

Gene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy.- Mini-Blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans.- Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans.- Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatumi.- Targeted Gene Disruption in Cryptococcus neoformans Using Double Joint PCR with Split Dominant Selectable Markers.- Multiple Gene Deletion in Cryptococcus neoformans Using the Cre--lox System.- Gene Disruption in Aspergillus fumigatus Using a PCR-Based Strategy and In Vivo Recombination in Yeast.- Targeted Gene Deletion in Aspergillus fumigatus Using the Hygromycin-Resistance Split-Marker Approach.- Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers.- RNAi-Based Gene Silencing Using a GFP Sentinel System in Histoplasma capsulatum.- RNA Interference in Cryptococcus neoformans.- Gene Knock-Down in Paracoccidioides brasiliensis Using Antisense RNA.- Tetracycline-Inducible Gene Expression in Candida albicans.- Galactose-Inducible Promoters in Cryptococcus neoformans var. grubii.- Modular Gene Over-Expression Strategies for Candida albicans.- Interactions Between Macrophages and Cell-Wall Oligosaccharides of Candida albicans.- Murine Bone Marrow-Derived Dendritic Cells and T Cell Activation by Candida albicans.- Phagocytosis and Intracellular Killing of Candida albicans by Murine Polymorphonuclear Neutrophils.- Human Oral Keratinocytes: A Model System to Analyze Host-Pathogen Interactions.- Simple Assays for Measuring Innate Interactions with Fungi.- Binding and Uptake of Candida albicans by Human Monocyte-Derived Dendritic Cells.-Immune Responses to Candida albicans in Models of In Vitro Reconstituted Human Oral Epithelium.- Analysis of Host-Cell Responses by Immunoblotting, ELISA, and Real-Time PCR.- In Vitro Model of Invasive Pulmonary Aspergillosis in the Human Alveolus.- Biofilm Formation Studies in Microtiter Plate Format.- Transcript Profiling Using ESTs from Paracoccidioides brasiliensis in Models of

Infection.- Laser Capture Microdissection of Candida albicans from Host Tissue.- Isolation and Amplification of Fungal RNA for Microarray Analysis from Host Samples.- Cytokine Measurement Using Cytometric Bead Arrays (CBA).- Transcript Profiling of the Murine Immune Response to Invasive Aspergillosis.- Caenorhabditis elegans: A Nematode Infection Model for Pathogenic Fungi.- Drosophila melanogaster as a Model Organism for Invasive Aspergillosis.- Galleria mellonella as a Model for Fungal Pathogenicity Testing.- Embryonated Chicken Eggs as Alternative Infection Model for Pathogenic Fungi.- Mouse Intravenous Challenge Models and Applications.- A Nebulized Intra-Tracheal Rat Model of Invasive Pulmonary Aspergillosis.- Invasive Models of Histoplasmosis.- Murine Model of Concurrent Oral and Vaginal Candida albicans Colonization.- A Luciferase Reporter for Gene Expression Studies and Dynamic Imaging of Superficial Candida albicans Infections.- Modeling of Fungal Biofilms Using a Rat Central-Vein Catheter.- Orogastrointestinal Model of Mucosal and Disseminated Candidiasis.- A Non-Lethal Murine Cutaneous Model of Invasive Aspergillosis.

Reviews

From the book reviews:“The book ‘Host-fungus Interactions’ assembles methods and protocols for studying gene disruption, modulation of gene expression, host and fungal responses to infection and several model systems to analyze the host-fungus interactions. The book comprises of 8 parts with 42 chapters and an index at the end of the book. … A useful tool for medical microbiologists, immunologists, geneticists, biotechnologists and students studying pharmacology and pathogenicity testing.” (Melvina D’souza and Kevin D. Hyde, Fungal Diversity, December, 2014)