Table of Contents

ENU Based Phenotype Driven Screening.- Detection and Quantification of Cytokines and Other Biomarkers.- FLOW CYTOMETRY ANALYSIS OF CELL CYCLING AND PROLIFERATION IN MOUSE HEMATOPOIETIC STEM AND PROGENITOR CELLS.- Flow Cytometry Measurement of Bone Marrow Perfusion in the Mouse and Sorting of Progenitors and Stems Cells According to Position Relative to Blood Flow in Vivo.- Analyzing Cell Death Events in Cultured Leukocytes.- Towards a four-dimensional view of neutrophils.- Isolation of Human and Mouse Neutrophils Ex vivo and In vitro.- MEASUREMENT OF OXIDATIVE BURST IN NEUTROPHILS.- Measurement of Neutrophil Elastase, Proteinase 3 and Cathepsin G Activities using Intramolecularly Quenched Fluorogenic Substrates.- The Macrophage.- Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice.- Generation of Mouse Bone Marrow-derived Macrophages.- Isolation and Differentiation of Monocytes-macrophages from Human Blood.- In Vitro Measurement of Phagocytosis and Killing of Cryptococcus neoformans by Macrophages.- Measuring the Inflammasome.- Arginine and Macrophage Activation.- Immunodetection of Granzyme B Tissue Distribution and Cellular Localisation.- Detection of Human and Mouse Granzyme B Activity in Cell Extracts.- T cell Transfer Model of Colitis: A Great Tool to Assess the Contribution of T cells in Chronic Intestinal Inflammation.- Measurement of Nitrite in Urine by Gas Chromatography-Mass Spectrometry.