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Mass Spectrometry for the Clinical Laboratory


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Accessible guide to mass spectrometry and the evaluation, validation, and troubleshooting of the most common assays in clinical pathology labs

Table of Contents

1. Mass spectrometry in clinical lab: determining the need and avoiding pitfalls 2. Application specific implementation of mass spectrometry platform 3. Sample preparation techniques for mass spectrometry in clinical lab: 4. Design of Validation and QC and Compliance 5. Best Practices for routine operation of clinical mass spectrometry assays 6. Toxicology: LCMS 7. Toxicology: GCMS 8. Therapeutic drug monitoring 9. Vitamin D and analogs 10. Steroid hormones 11. Thyroid hormones 12. Bacterial identification: MALDI TOF 13. High Resolution Accurate Mass (HRAM) Mass Spectrometry 14. Evolving platforms for clinical mass spectrometry (POCT, paper spray, TOF, etc)

About the Author

Dr. Nair received his PhD in 1998 under the advice of Prof. Vicki Wysocki at Virginia Commonwealth University, Richmond, VA with specialization in the study of gas phase dissociation of biomolecules in a mass spectrometer. Since then after an NRC postdoctoral fellowship at the US Army, Aberdeen Proving Grounds, MD he has worked for over a decade in the industry that include the tenures as senior scientist at Applied Biosysems where he participated in the development of novel mass spectrometric instrumentation for biotechnology applications and at Perkin Elmer where he was involved in the development and validation of IVD kits for new born screening and clinical lab testing applications. After completion of fellowship from the Department of Lab Medicine at the University of Washington, Seattle, he received his board certification in Clinical Chemistry by the American Board of Clinical Chemistry in 2014, and is a Fellow of the National Academy of Clinical Biochemistry. After a brief stint as a consultant to toxicology labs, he is currently employed as the technical director at the Boston Heart Diagnostics, Framingham, MA.Dr. Nair has co-authored several peer reviewed articles, a patent and served as a consulting editor for a med tech text book published by Elsevier. William Clarke received his PhD in Analytical Chemistry from the University of Nebraska in Lincoln in 2000, followed by a post-doctoral fellowship in Clinical Chemistry at the Johns Hopkins School of Medicine, ending in 2002. In addition, he received an MBA focused on Medical Services Management from the Carey School of Business at Johns Hopkins in 2007. He is an Associate Professor in the Department of Pathology, as well as the director of both Point-of-Care Testing and Clinical Toxicology for The Johns Hopkins Hospital. Dr. Clarke is board certified in Clinical Chemistry by the American Board of Clinical Chemistry, and is a Fellow of the National Academy of Clinical Biochemistry. Dr. Clarke has published, as author or co-author, over 100 peer-reviewed manuscripts and book chapters. He is the Editor of the book Contemporary Practice in Clinical Chemistry, and the Co-Editor-in-Chief for the journal Practical Laboratory Medicine.

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