Table of Contents

1. Using QSAR Models to Predict Mitochondrial Targeting by Small Molecule Xenobiotics within Living Cells

Richard W. Horobin

2. DQAsomes as the Prototype of Mitochondria-targeted Pharmaceutical Nanocarriers: An Update

Volkmar Weissig, Maria Lozoya, Nusem Yu, and Gerard G.M. D'Souza

3. Synthesis and Characterization of Mitochondria-Targeted Triphenyltriphenylphosphonium Bolaamphiphiles

Francesca Ceccacci, Simona Sennato, Edoardo Rossi, Raffaele Proroga, Stefano Sarti, Marco Diociaiuti, Stefano Casciardi, Valentina Mussi, Alessia Ciogli, Federico Bordi, Giovanna Mancini, and Cecilia Bombelli

4. Synthesis and Evaluation of ¹⁸F-labeled Fluoroalkyl Triphenylphosphonium Salts as Mitochondrial Voltage Sensors in PET Myocardial Imaging

Dong-Yeon Kim and Jung-Joon Min

5. Novel Mitochondria-Targeted Triphenylphosphonium Conjugates of Linear beta-Phosphorylated Nitrones: Preparation, ³¹P NMR Mitochondrial Distribution, EPR Spin Trapping Reporting, and Site-Directed Anti-Apoptotic Properties

Consuelo Petrocchi, Sophie Thetiot-Laurent, Marcel Culcasi, and Sylvia Pietri

6. Insights on Targeting Small Molecules to the Mitochondrial Matrix and the Preparation of MitoB and MitoP as exomarkers of Mitochondrial Hydrogen Peroxide

Andrew G. Cairns, Stephen J. McQuaker, Michael P. Murphy, and Richard C. Hartley

7. Synthesis of Triphenylphosphonium Phospholipid Conjugates for the Preparation of Mitochondriotropic Liposomes

Parul Benien, Mohammed Almuteri, Shrey Shah, Mark Boehlke, Ahmed Mehanna, and Gerard G. M. D'Souza

8. Imaging Mitochondrial Hydrogen Peroxide in Living Cells

Alexander R. Lippert, Bryan C. Dickinson, and Elizabeth J. New

9. Synthesis and Testing Of Novel Isomeric Mitochondriotropic Derivatives of Resveratrol and Quercetin

Lucia Biasutto, Andrea Mattarei, and Cristina Paradisi

10. Bridging the Gap between Nature and Antioxidant Setbacks: Delivering Gallic Acid to Mitochondria

Fernando Cagide, Catarina Oliveira, Jose Teixeira, Paulo J. Oliveira, and Fernanda Borges

11. Liposomal Delivery of Cyclocreatine Impairs Cancer Cell Bioenergetics Mediating Apoptosis

Samayita Ganguly and Tamer Elbayoumi

12. Mt-fura-2, a ratiometric Mitochondria-targeted Ca²⁺ sensor. Determination of spectroscopic properties and Ca²⁺ Imaging Assays

Andrea De Nadai, Nicola Vajente, Diana Pendin, and Andrea Mattarei

13. Sequence-Specific Control of Mitochondrial Gene Transcription using Programmable Synthetic Gene Switches called MITO-PIPs

Takuya Hidaka, Hiroshi Sugiyama, and Ganesh N. Pandian

14. Targeting the Mitochondrial Genome via a MITO-Porter: Evaluation of mtDNA and mtRNA Levels and Mitochondrial Function

Yuma Yamada and Hideyoshi Harashima

15. mTRIP, an Imaging Tool to Investigate Mitochondrial DNA dynamics in Physiology and Disease at the Single-Cell Resolution

Laurent Chatre and Miria Ricchetti

16. Development of Mitochondria-Targeted Imaging Nanoplatforms by Incorporation of Fluorescent Carbon Quantum Dots

Ye Zhang and Hong Bi

17. Norbormide-based Probes and their Application for Mitochondrial Imaging in Drosophila Melanogaster

Alessia Forgiarini , Zifei Wang , Sergio Bova, Margaret Anne Brimble, Brian Hopkins ³, David Rennison, and Genny Orso

18. Live-cell Assessment of Reactive Oxygen Species Levels Using Dihydroethidine

Sander Grefte and Werner J.H. Koopman

19. Protein Supercomplex Recording In Living Cells Via Position-Specific Fluorescence Lifetime Sensors

Bettina Rieger and Karin B. Busch

20. Identification of Peroxynitrite by Profiling Oxidation and Nitration Products from Mitochondria-Targeted Arylboronic Acid

Jacek Zielonka, Adam Sikora, Radoslaw Podsiadly, Micael Hardy, and B. Kalyanaraman

21. Mitochondrial Coenzyme Q10 Determination via Isotope Dilution Liquid Chromatography Tandem Mass Spectrometry

Erik Hedman and Outi Itkonen

22. Janus-type Mesoporous Silica Nanoparticles for Sequential Tumoral Cell and Mitochondria Targeting

Maria Rocio Villegas, Victoria Lopez, Veronica Rodriguez-Garcia, Alejandro Baeza, and Maria Vallet-Regi

23. Split Green Fluorescent Protein-Based Contact Site Sensor (SPLICS) for Heterotypic Organelle Juxtaposition as Applied to ER - Mitochondria Proximities

Tito Cali and Marisa Brini

24. Qualitative Characterization of the Rat liver Mitochondrial Lipidome using All Ion Fragmentation on an Exactive Benchtop Orbitrap MS

Irina G. Stavrovskaya, Rose M. Gathungu, Susan S. Bird, and Bruce S. Kristal

25. Live Imaging of Mitochondria in Kidney Tissue

Milica Bugarski, Susan Ghazi, and Andrew M Hall

26. Time-resolved Imaging of Mitochondrial Flavin Fluorescence and Its Applications for Evaluating the Oxidative State in Living Cardiac Cells

Marcek Chorvatova A., Cagalinec M., and Chorvat D Jr.

27. Mito-SinCe² Approach to Analyze Mitochondrial Structure-Function In Single Cells

Spurlock B and Mitra K

28. Computer-aided Prediction of Protein Mitochondrial Localization

Pier Luigi Martelli, Castrense Savojardo, Piero Fariselli, Giacomo Tartari, and Rita Casadio