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Molecular Diagnostic PCR Handbook
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Table of Contents

Preface. Acknowledgements. Chapter One: Background. Chapter Two: PCR - the basic reaction. Chapter Three: Amplicon detection, analysis and PCR evaluation. Chapter Four: Limitations and Troubleshooting. Chapter Five: Specific types of PCR assays. Chapter Six: The PCR laboratory. Chapter Seven: Disease diagnosis using PCR specific procedures for important veterinary pathogens. Appendices. Technical. Information and general protocols. Appendix A: Thermal cyclers. Appendix B: Basic protocol for agarose gel electrophoresis. Appendix C: Using nucleic acid hybridisation in order to verify the integrity of a PCR amplicon. Appendix D: Digestion of DNA with restriction endonucleases and electrophoresis of the restriction fragments. Appendix E: DNA sequence analysis. Appendix F: An example of a RT-PCR protocol. Appendix G (i): Calibration and Maintenance of Mechanical Pipettes. Appendix G (ii): Standard Operating Procedure for using micropipettes. Appendix H: Standard Operating Procedure for using clocks and timers. Appendix I: Theoretical background to pH, buffers and concentration of solutions. Appendix J: General buffer and stock solutions. Appendix K: General reagents and suppliers. Appendix L: Bioinformatics. Original Papers. Glossary. Index.

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