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Nanopore Technology
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Table of Contents

Part I: Design and Preparation of Biological Based Nanopores 1. Preparation of Fragaceatoxin C (FraC) Nanopores

Natalie Lisa Mutter, Gang Huang, Nieck van der Heide, Florian Leonardus Rudolfus Lucas, Nicole Stephanie Galenkamp, Giovanni Maglia, and Carsten Wloka

2. Preparation of Cytolysin A (ClyA) Nanopores

Nicole Stephanie Galenkamp, Veerle Van Meervelt, Natalie Lisa Mutter, Nieck van der Heide, Carsten Wloka, and Giovanni Maglia

3. Building Synthetic Transmembrane Peptide Pores

Kozhinjampara R. Mahendran

4. Design and Assembly of Membrane-Spanning DNA Nanopores

Kerstin Goepfrich and Ulrich F. Keyser

Part II: Single Molecule Detection and Analysis of Protein Analytes 5. Determining the Orientation of Porins in Planar Lipid Bilayers

Sandra A Ionescu, Sejeong Lee, and Hagan Bayley

6. Revelation of Function and Inhibition of Wza through Single Channel Studies

Lingbing Kong

7. Protein Analyte Sensing with an Outer Membrane Protein G (OmpG) Nanopore

Monifa A.V. Fahie, Bib Yang, Christina M. Chisholm, and Min Chen

8. Nanopore Enzymology to Study Protein Kinases and their Inhibition by Small Molecules

Leon Harrington, Leila T. Alexander, Stefan Knapp, and Hagan Bayley

9. A Selective Activity-Based Approach for Analysis of Enzymes with an OmpG Nanopore

Monifa A.V. Fahie, Bach Pham, Fanjun Li, and Min Chen

10. Oligonucleotide-Directed Protein Threading through a Rigid Nanopore

Garbine Celaya and David Rodriguez Larrea

11. Unfolding and Translocation of Proteins through an Alpha-Hemolysin Nanopore by ClpXP

Jeff Nivala, Logan Mulroney, Qing Luan, Robin Abu-Shumays, and Mark Akeson

Part III: Computational Analysis of Nanopore Behavior 12. Simulation of pH-Dependent, Loop-Based Membrane Protein Gating Using Pretzel

Alan Perez-Rathke, Monifa A.V. Fahie, Christina M. Chisholm, Min Chen, and Jie Liang

13. Free Energy Minimization for Vesicle Translocation through a Narrow Pore

Hamid R. Shojaei, Ahad Khaleghi Ardabili, and Murrugappan Muthukumar

Part IV: Droplet Interface Bilayer System 14. Single Ion-Channel Analysis in Droplet Interface Bilayer

Arash Manafirad

15. Continuous and Rapid Solution Exchange in a Lipid Bilayer Perfusion System Based on Droplet-Interface Bilayer

En-Hsin Lee

16. Protein Transport Studied by a Model Asymmetric Membrane Army Arranged in a Dimple Chip

Xin Li and Min Chen

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