Table of Contents

3’ end Formation and Regulation of Eukaryotic mRNAs.- Poly(A) Polymerase-based Poly(A) Length assay.- Using Klenow Mediated Extension to Measure Poly(A)-tail Length and Position in the Transcriptome.- Detection of Uridylated mRNAs.- Cytoplasmic Polyadenylation Assays.- In vitro Analysis of Transcriptional Activators and Polyadenylation.- Fluorescence Detection of Polyadenylation Reaction through the Coordination of Adensoine2-coralyne-adensoine2.- Small molecule recognition of poly(A).-The Stem-loop Luciferase assay for Polyadenylation (SLAP) Method for Determining CstF-64-dependent Polyadenylation Activity.- Accurate Mapping of Cleavage and Polyadenylation Sites by 3' Region Extraction and Deep Sequencing.- Polyadenylation Site Prediction using PolyA-iEP Method.- Detecting alternative polyadenylation from Microarray Data.- Quantitative Polyadenylation Site Mapping with Single-molecule Direct RNA Sequencing.- RHAPA: A New Method to Quantify Alternative Polyadenylation.- Multiplex Analysis of PolyA-linked Sequences (MAPS): An RNA-seq Strategy to Profile poly(A+) RNA.- Global and Quantitative Profiling of Polyadenylated RNAs using PAS-seq.- High-throughput Sequencing of RNA Isolated by Crosslinking and Immunoprecipitation (HITS-CLIP) to Determine Sites of Binding of CstF-64 on Nascent RNAs.- Polyadenylation in Bacteria and Organelles.- In vivo Analysis of Polyadenylation in Prokaryotes.- Polyadenylation of RNA in E. coli: RNA Polymerase-associated (rA)n-synthetic Activities.- Analysis of the Human Mitochondrial Transcriptome using Directional Deep sequencing and Parallel Analysis of RNA Ends.- Measurement of Mitochondrial RNA Stability by Metabolic Labeling of Transcripts with 4-Thiouridine .- Deadenylation and its Regulation in Eukaryotic Cells.- Assaying mRNA Deadenylation in vitro.- Assaying mRNA Deadenylation in vivo.- In vitro Analysis of RNA Degradation Catalyzed by Deadenylase Enzymes.-microRNA-mediated Deadenylation in a Mammalian Cell-free System.- Quantitative Analysis of Deadenylation-independent mRNA Decay by a Modified MBRACE Assay.