Table of Contents

List of Contributors vii

Preface xiii

1 Isolation and Characterization of Human Embryonic Stem Cells and Future Applications in Tissue Engineering Therapies 3
Christian Unger, James Hackland, David Preskey and Harry Moore

2 Epigenetics, Stem Cell Pluripotency and Differentiation 27
Maria J. Barrero

3 Stem Cell Niche and Microenvironment 45
Marcio Alvarez-Silva

4 Haematopoietic Stem Cells in Therapy 65
Jos Domen and Jignesh Dalal

5 Isolation and Identification of Neural Stem/Progenitor Cells 85
Loic P. Deleyrolle and Brent A. Reynolds Part II Cancer Stem Cells 101

6 The Role of Epithelial-Mesenchymal Transition in Cancer Metastasis 103
Paul C. McDonald and Shoukat Dedhar

7 Regulation of Breast Cancer Stem Cells by Mesenchymal Stem Cells in the Metastatic Niche 123
Fayaz Malik, Hasan Korkaya, Shawn G. Clouthier and Max S. Wicha

8 Isolation and Identification of Neural Cancer Stem/Progenitor Cells 145
David Bakhshinyan, Maleeha Qazi, Neha Garg, Chitra Venugopal,

9 Colon Stem Cells in Colorectal Cancer 169
Varun V. Prabhu, Wafik S. El-Deiry and Niklas Finnberg

10 Prostate Cancer and Prostate Cancer Stem Cells 193
Magdalena E. Buczek, Jerome C. Edwards and Tarik Regad

11 Stem Cells and Pancreatic Cancer 213
Audrey M. Hendley and Jennifer M. Bailey

12 NANOG in Cancer Development 231
Bigang Liu and Dean G. Tang

13 Liver Cancer Stem Cells and Hepatocarcinogenesis 253
Hirohisa Okabe, Hiromitsu Hayashi, Takatsugu Ishimoto, Kosuke Mima, Shigeki Nakagawa, Hideyuki Kuroki, Katsunori Imai, Hidetoshi Nitta, Daisuke Hashimoto, Akira Chikamoto, Takatoshi Ishiko, Toru Beppu and Hideo Baba

14 Basic Science of Liver Cancer Stem Cells and Hepatocarcinogenesis 273
Katherine S. Koch and Hyam L. Leffert

15 Cancer Stem Cell Biomarkers 305
Stefano Zapperi and Caterina A.M. La Porta

16 Interatomic Analysis of the Stem Cell Marker NANOG in a Prostate Cancer Setting 317
Kiran Mall and Graham Ball

Index 353

About the Author

Tarik Regad, The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK Thomas J. Sayers, Leidos Biomedical Research Inc., and the Cancer and Infl ammation Program, Frederick National Laboratory for Cancer Research, Frederick, MD, USA Robert C. Rees, The John van Geest Cancer Research Centre, Nottingham Trent University, Nottingham, UK