Table of Contents

Protein Purification: An Overview.- Establishment of a Design Space for Biopharmaceutical Purification Processes Using DoE.- High Throughput Process Development: I. Process Chromatography.- High Throughput Process Development: II. Membrane Chromatography.- Media Selection in Ion Exchange Chromatography in a Single Microplate.- High Throughput Screening of Dye-Ligands for Chromatography.- Measurement of Uptake Curves and Adsorption Isotherms by Automated Microscale Chromatography Pipette Tips.- Recovery of Recombinant Proteins from Plants Using Aqueous Two-Phase Partitioning Systems: An Outline.- Aqueous Two-Phase Systems Strategies for the Recovery of Proteins from Plants.- Aqueous Two Phase Assisted Precipitation of Proteins: A Platform for Isolation of Process Related Impurities from Therapeutic Proteins.- Simultaneous Purification and Refolding of Proteins by Affinity Precipitation and Macro-(Affinity Ligand) Facilitated Three Phase Partitioning (MLFTPP).- Co-Expression and Co-Purification of Antigen-Antibody Complexes in Bacterial Cytoplasm and Periplasm.- Immunoglobulin Purification by Caprylic Acid.- Affinity Tags in Protein Purification and Peptide Enrichment: An Overview.- Expression and Purification of Large Active GST Fusion Enzymes.- Synthetic Ligand Affinity Chromatography. Purification of Human Serum Albumin and Related Fusion Proteins.- Zbasic - A Purification Tag for Selective Ion-Exchange Recovery.- An Orthogonal Fusion Tag for Efficient Protein Purification.- Phage Display of Engineered Binding Proteins.- Biomimetic Affinity Ligands for Protein Purification.- Synthesis and Application of Dye-Ligand Affinity Adsorbents.- Peptide Affinity Chromatography Based on Combinatorial Strategies for Protein Purification.- Affinity Chromatography of Proteins on Monolithic Columns.- Sample Displacement Batch Chromatography of Proteins.- Analysis of Host-Cell Proteins in Biotherapeutic Proteins by LC/MS Approaches.- Preparation of Monolithic Affinity Media for Nano-Liquid Chromatography Applications.- Proteomic Analysis of Complex Protein Samples by MALDI TOF Mass Spectrometry.- Modern Bioanalysis of Proteins by Electrophoretic Techniques.- Protein Structure Validation and Analysis with X-Ray Crystallography.- Measuring Binding Constants of His-Tagged Proteins using Affinity Chromatography and Ni-NTA Immobilized Enzymes.- Stabilization of Therapeutic Proteins in Aqueous Solutions and Freeze-Dried Solids: An Overview.- Stabilization of Protein by Freeze-Drying in the Presence of Trehalose: A Case Study of Tubulin.- G Protein-Coupled Receptor Expression and Purification.-  (Hyper)thermophilic Enzymes: Production and Purification.- Affinity Chromatography for Antibody Purification.- Screening and Purification of Recombinant Lignocellulolytic Enzymes.- Purification of PEGylated Proteins, with the Example of PEGylated lysozyme and PEGylated scFv.- One-Step Purification ofGlutamate decarboxylase from E. coli Using Aqueous Two Phase System.