Table of Contents

Preface to The Third Edition vii Preface to The Second Edition ix Preface to The First Edition xi Contributors xiii Part I Introduction 1 1 Introduction to Protein Purification 3 Bo Ersson, Lars Ryden, and Jan-Christer Janson Part II Chromatography 23 2 Introduction to Chromatography 25 Jan-Christer Janson and Jan Ake Joensson 3 Gel Filtration: Size Exclusion Chromatography 51 Lars Hagel 4 Ion Exchange Chromatography 93 Evert Karlsson and Irwin Hirsh 5 High-Resolution Reversed-Phase Chromatography of Proteins 135 Sylvia Winkel Pettersson 6 Hydrophobic Interaction Chromatography 165 Kjell-Ove Eriksson and Makonnen Belew 7 Immobilized Metal Ion Affinity Chromatography 183 Lennart Kagedal 8 Covalent Chromatography 203 Francisco Batista-Viera, Lars Ryden, and Jan Carlsson 9 Affinity Chromatography 221 Francisco Batista-Viera, Jan-Christer Janson, and Jan Carlsson 10 Affinity Ligands from Chemical Combinatorial Libraries 259 Enrique Carredano and Herbert Baumann 11 Affinity Ligands from Biological Combinatorial Libraries 269 Per- Ake Nygren Part III Other Separation Methods and Related Techniques 279 12 Membrane Separations 281 Joachim K. Walter, Zuwei Jin, Maik W. Jornitz, and Uwe Gottschalk 13 Refolding of Inclusion Body Proteins from E. coli 319 Zhiguo Su, Diannan Lu, and Zheng Liu 14 Purification of PEGylated Proteins 339 Conan J. Fee and James M. Van Alstine Part IV Electrophoresis 363 15 Electrophoresis in Gels 365 Reiner Westermeier 16 Conventional Isoelectric Focusing in Gel Slabs and Capillaries and Immobilized pH Gradients 379 Pier Giorgio Righetti, Elisa Fasoli, and Sabina Carla Righetti 17 Two-Dimensional Electrophoresis in Proteomics 411 Reiner Westermeier and Angelika Goerg 18 Protein Elution and Blotting Techniques 441 Reiner Westermeier 19 Capillary Electrophoretic Separations 451 Wolfgang Thormann Part V Separation Method Optimization 487 20 High Throughput Screening Techniques in Protein Purification 489 Karol M. Lacki and Eggert Brekkan Index 507

About the Author

JAN-CHRISTER JANSON is Professor Emeritus at the Department of Physical and Analytical Chemistry, Uppsala University, Uppsala, Sweden.