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Quality in Laboratory Hemostasis and Thrombosis


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Table of Contents

Contributors, vii Foreword, x Frits R. Rosendaal Preface, xii 1 General Quality Planning in the Hemostasis Laboratory, 1 John D. Olson 2 Hemostasis Test Validation, Performance and Reference Intervals, 9 Richard A. Marlar 3 International Standards in Hemostasis, 19 Trevor W. Barrowcliffe 4 Sample Integrity and Preanalytical Variables, 31 Dorothy M. Adcock 5 Internal Quality Control in the Hemostasis Laboratory, 43 Steve Kitchen, F. Eric Preston, John D. Olson 6 External Quality Assessment in Hemostasis: Its Importance and Signifi cance, 51 F. Eric Preston, Steve Kitchen, Alok Srivastava 7 Initial Evaluation of Hemostasis: Reagent and Method Selection, 63 Wayne L. Chandler 8 Point-of-Care Testing in Hemostasis, 72 Chris Gardiner, Samuel Machin, Ian Mackie 9 Assay of Factor VIII and Other Clotting Factors, 81 Steve Kitchen, F. Eric Preston 10 Application of Molecular Genetics to the Investigation of Inherited Bleeding Disorders, 90 Stefan Lethagen, Marianne Schwartz, Lars Bo Nielsen 11 Standardization of d-dimer Testing, 99 Guido Reber, Philippe de Moerloose 12 Diagnostic Assessment of Platelet Function, 110 Paquita Nurden, Alan Nurden 13 Laboratory Evaluation of von Willebrand Disease: Phenotypic Analysis, 125 Emmanuel J. Favaloro 14 Laboratory Analysis of von Willebrand Disease: Molecular Analysis, 137 Anne Goodeve, Ian Peake 15 Dilemmas in Heritable Thrombophilia Testing, 147 Isobel D. Walker, Ian Jennings 16 Evaluation of Antiphospholipid Antibodies, 160 Michael Greaves 17 Monitoring Heparin Therapy, 170 Marilyn Johnston 18 Monitoring Oral Anticoagulant Therapy, 179 Armando Tripodi 19 Monitoring New Anticoagulants, 190 Elaine Gray, Trevor W. Barrowcliffe 20 Detecting and Quantifying Functional Inhibitors in Hemostasis, 198 Bert Verbruggen, Irena Novakova, Waander van Heerde Index, 209

About the Author

Eric Preston MD, FRCP, FRCPath Professor Preston is Emeritus Professor of Haematology at the University of Sheffield since 2000, having been Professor of Haematology in the same University from 1986 - 2000. He was Director of the Sheffield Haemostasis and Thrombosis Centre from 1973 - 2000. He is a past President of the British Society for Haematology and also the British Society for Haemostasis and Thrombosis. During the period 1992 - 2005 he was Director of the UK National External Quality Assessment Scheme (UKNEQAS) for Blood Coagulation. He continues as Director of the WHO International External Quality Assessment Program for Blood Coagulation testing for Developing Countries and also as Director of the World Federation of Hemophilia (WFH) Blood Coagulation EQA program. Professor Preston has been a co-author on more than 250 publications in peer reviewed journals in the field of hemostasis and thrombosis. He is currently vice chairman of the newly formed international group devoted to EQA in hemostasis and thrombosis EQATH). Steve Kitchen PhD Dr Steve Kitchen is currently the Scientific Director of the UK National External Quality Assessment Scheme for Blood Coagulation and is the Head Scientist for the WHO and World Federation of Haemophilia EQA programmes . He is the head scientist at the Sheffield Haemostasis and Thrombosis Centre. He has 25 years experience in the field of laboratory testing in haemostasis with a long standing interest in standardisation and has been involved in drawing up guidelines for a number of National and International bodies in the field. John D. Olson, MD, PhD Dr Olson is Professor of Pathology at the University of Texas Health Science Center at San Antonio and Director of Clinical Laboratories for the University Health Systems. He has had a continuing interest in haemostasis and thrombosis that has spanned his career of more than 30 years. Recently, he was the Chair of the Coagulation Resource Committee (1999 to 2004) of the College of American Pathologists. This committee a the largest external quality assurance program in haemostasis in North America. He has spoken at national and international meetings on the topic of quality in haemostasis. He is currently the chair of a newly formed international group of programs devoted to external quality assurance in haemostasis and thrombosis (EQATH).


"There are particularly useful chapters covering the monitoring of heparin and oral anticoagulation. The book is pretty up to date and even covers the monitoring of new anticoagulants." ( The Bulletin of the Royal College of Pathologists, July 2010)

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