Table of Contents

Characterization of RNA-Protein Interactions: Lessons from Two RNA-Binding Proteins, SRSF1 and SRSF2.- Identification of mRNA-Interacting Factors by MS2-TRAP (MS2-Tagged RNA Affinity Purification).- Biotin-Streptavidin Affinity Purification of RNA-Protein Complexes Assembled In Vitro.- Detecting RNA-Protein Interaction Using End-Labeled Biotinylated RNA Oligonucleotides and Immunoblotting.- Purification of RNA-Protein Splicing Complexes Using a Tagged Protein from In Vitro Splicing Reaction Mixture.- Loading of Argonaute Protein with Small Duplex RNA in Cellular Extracts.- In Vitro Analysis of Ribonucleoprotein Complex Remodeling and Disassembly.- Single-Turnover Kinetics of Methyl Transfer to tRNA by Methyltransferases.- Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles.- Northwestern Blot Analysis: Detecting RNA-Protein Interaction After Gel Separation of Protein Mixture.- Measuring mRNA Translation by Polysome Profiling.- Genome-Wide Profiling of RNA-Protein Interactions Using CLIP-Seq.- Identification of Endogenous mRNA-Binding Proteins in Yeast Using Crosslinking and PolyA Enrichment.- Ribo-Proteomics Approach to Profile RNA-Protein and Protein-Protein Interaction Networks.- Detection of Protein-Protein Interaction Within an RNA-Protein Complex Via Unnatural-Amino-Acid-Mediated Photochemical Crosslinking.- Evolution of Cell-Type-Specific RNA Aptamers Via Live Cell-Based SELEX.- mCarts: Genome-Wide Prediction of Clustered Sequence Motifs as Binding Sites for RNA Binding Proteins.- Design of RNA-Binding Proteins: Manipulate Alternative Splicing in Human Cells with Artificial Splicing Factors.- Informational Suppression to Probe RNA:RNA Interactions in the Context of Ribonucleoproteins: U1 and 5’ Splice-Site Base-Pairing.- Analysis of Alternative Pre-RNA Splicing in the Mouse Retina Using a Fluorescent Reporter.