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Rat Genomics
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The Rat: A Model Used in Biomedical Research.- Genetic Mapping and Positional Cloning.- Sequencing of the Rat Genome and Databases.- Design of Expression Cassettes for the Generation of Transgenic Animals (Including Insulators).- Inducible and Conditional Promoter Systems to Generate Transgenic Animals.- Generation of Transgenic Rats by Microinjection of Short DNA Fragments.- Generation of Transgenic Rats Using YAC and BAC DNA Constructs.- The Use of Lentiviral Vectors to Obtain Transgenic Rats.- Generation of Transgenic Rats by Ooplasmic Injection of Sperm Cells Exposed to Exogenous DNA.- Procedures for Somatic Cell Nuclear Transfer in the Rat.- ENU Mutagenesis to Generate Genetically Modified Rat Models.- Establishment of Embryonic Stem Cells from Rat Blastocysts.- Derivation, Culture, and In vivo Developmental Capacity of Embryonic Cell Lines from Rat Blastocysts.- Interference RNA for In vivo Knock-Down of Gene Expression or Genome-Wide Screening Using shRNA.- Generation of Gene-Specific Mutated Rats Using Zinc-Finger Nucleases.- Application of Microarray-Based Analysis of Gene Expression in the Field of Toxicogenomics.- Generation of Congenic and Consomic Rat Strains.- Generation of Rat “Supersonic” Congenic/Conplastic Strains Using Superovulation and Embryo Transfer.- Analysis by Quantitative PCR of Zygosity in Genetically Modified Organisms.- A Restriction Enzyme-PCR-Based Technique to Determine Transgene Insertion Sites.- Cryopreservation and Orthotopic Transplantation of Rat Ovaries.- Techniques for In Vitro and In Vivo Fertilization in the Rat.- Rat Strain Repositories.- Neurobehavioral Tests in Rat Models of Degenerative Brain Diseases.- Rat Genomics Applied to Psychiatric Research.- Genomics Studies of Immune-Mediated Diseases Using the BN–LEW RatModel.- Rat Models of Cardiovascular Diseases.- Use of Rat Genomics for Investigating the Metabolic Syndrome.- Genomic Research in Rat Models of Kidney Disease.- Cancer Research in Rat Models.

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