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Rat Genomics
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1. The Rat: A Model Used In Biomedical Research Howard J. Jacob 2. Genetic Mapping and Positional Cloning Timothy J. Aitman, Enrico Petretto, and Jacques Behmoaras 3. Sequencing of the Rat Genome and Databases Elizabeth A. Worthey, Alexander J. Stoddard, and Howard J. Jacob 4. Design of Expression Cassettes for the Generation of Transgenic Animals (Including Insulators) Louis-Marie Houdebine 5. Inducible and Conditional Promoter Systems to Generate Transgenic Animals Yoji Hakamata and Eiji Kobayashi 6. Generation of Transgenic Rats by Microinjection of Short DNA Fragments Severine Menoret, Severine Remy, Claire Usal, Laurent Tesson, and Ignacio Anegon 7. Generation of Transgenic Rats Using YAC and BAC DNA Constructs Ri-ichi Takahashi and Masatsugu Ueda 8. The Use of Lentiviral Vectors to Obtain Transgenic Rats Severine Remy, Tuan Huy NGuyen, Severine Menoret, Laurent Tesson, Claire Usal, and Ignacio Anegon 9. Generation of Transgenic Rats by Ooplasmic Injection of Sperm Cells Exposed to Exogenous DNA Masumi Hirabayashi and Shinichi Hochi 10. Procedures for Somatic Cell Nuclear Transfer in the Rat Jean Cozzi, Eryao Wang, Christelle Jacquet, Alexandre Fraichard, Yacine Cherifi, and Qi Zhou 11. ENU Mutagenesis to Generate Genetically Modified Rat Models Ruben van Boxtel, Michael N. Gould, Edwin Cuppen, and Bart M. G. Smits 12. Establishment of Embryonic Stem Cells from Rat Blastocysts Masaki Kawamata and Takahiro Ochiya 13. Derivation, Culture, and in vivo Developmental Capacity of Embryonic Cell Lines from Rat Blastocysts Simon-Pierre Demers and Lawrence C. Smith 14. Interference RNA for in vivo Knock-Down of Gene Expression orGenome-Wide Screening Using shRNA Silvere Petit and Kader Thiam 15. Generation of Gene-Specific Mutated Rats Using Zinc-Finger Nucleases Aron M. Geurts, Gregory J. Cost, Severine Remy, Xiaoxia Cui, Laurent Tesson, Claire Usal, Severine Menoret, Howard J. Jacob, Ignacio Anegon, and Roland Buelow 16. Application of Microarray-Based Analysis of Gene Expression in the Field of Toxicogenomics Nan Mei, James C. Fuscoe, Edward K. Lobenhofer, and Lei Guo 17. Generation of Congenic and Consomic Rat Strains Dominique Lagrange and Gilbert J. Fournie 18. Generation of Rat 'Supersonic' Congenic/Conplastic Strains Using Superovulation and Embryo Transfer Vladimir Landa, Vaclav Zidek, and Michal Pravenec 19. Analysis by Quantitative PCR of Zygosity in Genetically Modified Organisms Laurent Tesson, Severine Remy, Severine Menoret, Claire Usal, and Ignacio Anegon 20. A Restriction Enzyme-PCR-Based Technique to Determine Transgene Insertion Sites Elizabeth C. Bryda and Beth A. Bauer 21. Cryopreservation and Orthotopic Transplantation of Rat Ovaries Martina Dorsch and Dirk Wedekind 22. Techniques for In Vitro and In Vivo Fertilization in the Rat Naomi Kashiwazaki, Yasunari Seita, Akiko Takizawa, Naoki Maedomari, Junya Ito, and Tadao Serikawa 23. Rat Strain Repositories Birger Voigt 24. Neurobehavioral Tests in Rat Models of Degenerative Brain Diseases Yvonne K. Urbach, Felix Bode, Huu Phuc Nguyen, Olaf Riess, and Stephan von Hoersten 25. Rat Genomics Applied to Psychiatric Research Marie-Pierre Moisan and Andre Ramos 26. Genomics Studies of Immune-Mediated Diseases Using the BN-LEW Rat Model Isabelle Bernard, Gilbert Fournie, and Abdelhadi

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