Part I: Genetic Engineering and Tissue Culture of Rice 1. Gene Assembly in Agrobacterium via Nucleic Acid Transfer Using Recombinase Technology (GAANTRY)
Leyla T. Hathwaik, James G. Thomson, and Roger Thilmony
2. TransGene Stacking II Vector System for Plant Metabolic Engineering and Synthetic BiologyQinlong Zhu and Yao-Guang Liu
3. Morphogenic Regulators and Their Application in Improving Plant TransformationSamson Nalapalli, Meral Tunc Ozdemir, Yuejin Sun, Sivamani Elumalai, and Qiudeng Que
4. A Rapid Method for Stably Transforming Rice SeedsSudheer Kumar and Neeti Sanan-Mishra
5. Agrobacterium tumefaciens-Mediated Transformation of Rice by Hygromycin Phosphotransferase (hptII) Gene Containing CRISPR/Cas9 VectorShuvobrata Majumder, Karabi Datta, and Swapan Kumar Datta
6. Genotype Independent Regeneration and Transformation Protocol for Rice CultivarsTanushri Kaul, Sonia Khan Sony, Nitya Meenakshi Raman, Khaled Fathy Motelb, and Jyotsna Bharti
Part II: Genome Editing and Targeted Integration in Rice 7. Rapid Vector Construction and Assessment of BE3 and Target-AID C to T Base Editing Systems in Rice ProtoplastsSimon Sretenovic, Changtian Pan, Xu Tang, Yong Zhang, and Yiping Qi
8. Genome Editing of Rice by CRISPR-Cas: End-to-End Pipeline for Crop ImprovementAmit Das, Pallavi Ghana, Bhojaraja Rudrappa, Rita Gandhi, Sresty Tavva Venkata, and Amitabh Mohanty
9. Single Base Editing Using Cytidine Deaminase to Change Grain Size and Seed Coat Color in RiceMy Vo Thi Tra, Xiaojia Yin, Ishita Bajal, Christian Paolo Balahadia, William Paul Quick, and Anindya Bandyopadhyay
10. Analysis of Off-Target Mutations in CRISPR-Edited Rice Plants Using Whole Genome SequencingGuanqing Liu, Yiping Qi, and Tao Zhang
11. Efficient Genome Editing in Rice Protoplasts Using CRISPR/CAS9 ConstructMartine Bes, Leo Herbert, Thibault Mounier, Anne-Cecile Meunier, Franz Durandet, Emmanuel Guiderdoni, and Christophe Perin
12. Single Transcript Unit CRISPR 2.0 Systems for Genome Editing in RiceXu Tang, Yiping Qi, and Yong Zhang
13. Improving a Quantitative Trait in Rice by Multigene Editing with CRISPR-Cas9Yesuf Teslim Yimam, Jianping Zhou, Sayed Abdul Akher , Xuelian Zheng,
Yiping Qi, and Yong Zhang
14. Rice Haploid Inducer Development by Genome EditingJuntao Liu, Dawei Liang, Li Yao, Ya Zhang, Chunxia Liu, Yubo Liu, Yanli Wang, Hongju Zhou, Timothy Kelliher, Xingping Zhang, and Anindya Bandyopadhyay
15. FLP-Mediated Site-Specific Gene Integration in RiceVibha Srivastava
16. Rice Gene Targeting by Homologous Recombination with Positive Negative Selection StrategyRie Terada and Zenpei Shimatani
Part III: Bioinformatics, Cellular, and Molecular Analysis of Rice 17. Prediction of Rice Transcription Start Sites Using TransPrise: A Novel Machine Learning ApproachStepan Pachganov, Khalimat Murtazalieva, Alexei Zarubin, Tatiana Taran, Duane Chartier, and Tatiana V. Tatarinova
18. Single Cell Type Specific RNA Isolation and Gene Expression Analysis in Rice Using Laser Capture Microdissection (LCM)-Based MethodVibhav Gautam, Sourav Chatterjee, and Ananda K. Sarkar
19. Immunolocalization Analysis of C4 Proteins in the Leaf Tissue of RiceHsiang-Chun Lin, Joanne Jerenice Anonuevo, Paul Quick, and Anindya Bandyopadhyay
20. Selection of Suitable Reference Genes for qRT-PCR Gene Expression Studies in RiceMeng Wang and Navreet K. Bhullar
21. Rice Protoplast Isolation and Transfection for Transient Gene Expression AnalysisJennylyn L. Trinidad, Toshisangba Longkumer, and Ajay Kohli
22. Identification and Analysis of Steroidogenic Acute Regulatory (StAR) Protein-Related Lipid Transfer (START) Domains in RiceSanjeet Kumar Mahtha, Ravikiran Purama, Renu Kumari, and Gitanjali Yadav
23. Assessing Rice Salinity Tolerance: From Phenomics to Association MappingNadia Al-Tamimi, Helena Oakey, Mark Tester, and Sonia Negrao
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