Table of Contents

 A Guide to Using STITCHER for Overlapping Assembly PCR Applications.- Synthetic Gene Design Using Codon Optimization On-Line (COOL).- Shuffle Optimizer: A Program to Optimize DNA Shuffling for Protein Engineering.- Simple Cloning by Prolonged Overlap Extension PCR with Application to the Preparation of Large-Size Random Gene Mutagenesis Library in Escherichia coli.- SpeedyGenes: Exploiting an Improved Gene Synthesis Method for the Efficient Production of Error-corrected, Synthetic Protein Libraries for Directed Evolution.- BASIC – A Simple and Accurate Modular DNA Assembly Method.- Enzymatic Synthesis of Single Stranded Clonal Pure Oligonucleotides.- Rapid Assembly of DNA via Ligase Cycling Reaction (LCR).- PaperClip: A Simple Method for Flexible Multi-part DNA Assembly.- The Polymerase Step Reaction (PSR) Method for Gene and Library Synthesis.- Clonetegration Using OSIP Plasmids – One Step DNA Assembly and Site Specific Genomic Integration in Bacteria.- Generation of DNA Constructs Using the Golden GATEway Cloning Method.- Gene Deletion by Synthesis in Yeast.- Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR).- Simultaneous Removal of Multiple DNA Segments by Polymerase Chain Reactions.- Rapid Construction of Recombinant Plasmids by QuickStep-Cloning.- Immobilized MutS-mediated Error Removal of Microchip-synthesized DNA.- Selection of Error-less Synthetic Genes in Yeast.