Table of Contents

Biopharmaceutical Proteins.- Expression of Antibody Fragments by Periplasmic Secretion in Escherichia coli.- Secretory Production of Therapeutic Proteins in Escherichia coli.- Expression of Recombinant LTB Protein in Marine vibrio VSP60.- Heterologous Gene Expression in Yeast.- Pharmaceutical Proteins From Methylotrophic Yeasts.- Expression of Human Papillomavirus Type 16 L1 in Baculovirus Expression Systems.- Large-Scale Transient Expression of Therapeutic Proteins in Mammalian Cells.- Site-Specific Integration for High-Level Protein Production in Mammalian Cells.- Production of Recombinant Therapeutic Proteins by Mammalian Cells in Suspension Culture.- Transgene Control Engineering in Mammalian Cells.- Fusion to Albumin as a Means to Slow the Clearance of Small Therapeutic Proteins Using the Pichia pastoris Expression System.- High-Throughput Recovery of Therapeutic Proteins from the Inclusion Bodies of Escherichia coli.- Isolation, Solubilization, Refolding, and Chromatographic Purification of Human Growth Hormone from Inclusion Bodies of Escherichia coli Cells.- Large-Scale Preparation of Factor VIIa from Human Plasma.- Purification of Clinical-Grade Monoclonal Antibodies by Chromatographic Methods.- Virus Elimination and Validation.- Virus Removal by Nanofiltration.- Determining Residual Host Cell Antigen Levels in Purified Recombinant Proteins by Slot Blot and Scanning Laser Densitometry.- Principles of Biopharmaceutical Protein Formulation.- Solid-State Protein Formulation.- Stabilization of Therapeutic Proteins by Chemical and Physical Methods.- Extraction and Characterization of Vaccine Antigens from Water-in-Oil Adjuvant Formulations.- Probing Reversible Self-Association of Therapeutic Proteins by Sedimentation Velocity in the Analytical Ultracentrifuge.- Biological Characterization of Pegylated Interferons.- Characterization of Interferon ?2B Pegylated via Carboxyalkylation.- Quantifying Recombinant Proteins and Their Degradation Products Using SDS-PAGE and Scanning Laser Densitometry.- Extraction and Denaturing Gel Electrophoretic Methodology for the Analysis of Yeast Proteins.- Characterization of Therapeutic Proteins by Membrane and In-Gel Tryptic Digestion.- Oligosaccharide Release and MALDI-TOF MS Analysis of N-Linked Carbohydrate Structures from Glycoproteins.- Capillary Electrophoresis of Carbohydrates Derivatized With Fluorophoric Compounds.- High-Throughput LC/MS Methodology for ?(1?3)Gal Determination of Recombinant Monoclonal Antibodies.- Carbohydrate Structural Characterization of Fas Ligand Inhibitory Protein.- Top-Down Characterization of Protein Pharmaceuticals by Liquid Chromatography/Mass Spectrometry.- Sample Preparation Procedures for High-Resolution Nuclear Magnetic Resonance Studies of Aqueous and Stabilized Solutions of Therapeutic Peptides.

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