1. Basic Investigations in Saccharomyces cerevisiae Brendan Curran and Virginia Bugeja2. Isolation of Yeast Nucleic Acids Michael Biss, Michelle Hanna and Wei Xiao3. Quantification of GFP Signals by Fluorescent Microscopy and Flow Cytometry Ting Wei and Heping Dai4. Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method R. Daniel Gietz5. Targeted Gene Deletion in Saccharomyces cerevisiae and Schizosaccharomyces pombe Johannes H. Hegemann, Sven Boris Heick, Jennifer Poehlmann, Markus Langen and Ursula Fleig6. See & Catch Method for Studying Protein Complexes in Yeast Cells: A Technique Unifying Fluorescence Microscopy and Mass Spectrometry Changhui Deng and Andrew N. Krutchinsky7. Yeast Two Hybrid Liquid Screening Elisa Donnard, Erica M. Queiroz, J. Miguel Ortega, and R. Daniel Gietz8. Targeted Mutagenesis of a Specific Gene in Yeast Likui Zhang, Alina Radziwon, and Linda J. Reha-Krantz9. Fluorescence Staining of Mitochondria for Morphology Analysis in S. cerevisiae Kai Stefan Dimmer 10. Assays for Autophagy I: the Cvt Pathway and Non-Selective Autophagy Wei-Pang Huang, Takahiro Shintani, and Zhiping Xie11. Assays for Autophagy II: Mitochondrial Autophagy Tomotake Kanki and Koji Okamoto12. Biochemically Characterizing the Subcellular Localization of Peroxisomal Proteins by Fractionation, Protease Protection and Carbonate Extraction Danielle Hagstrom and Changle Ma13. Yeast Survival and Growth Assays Xin Xu, Amanda Lambrecht, and Wei Xiao14. Spontaneous Mutagenesis Assay Susan Blackwell, Michelle Hanna, and Wei Xiao15. Mutational Specificity Analysis: Assay for Mutations in the Yeast SUP4-o Gene Bernard A. Kunz16. piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe Jun Li and Li-Lin Du17. Replicative and Chronological Life Span Assays Spike D. L. Postnikoff and Troy A. A. Harkness18. Detection of Protein Arginine Methylation in Saccharomyces cerevisiae Christopher Aa. Jackson and Michael C. Yu19. Detection of Protein Post-translational Modifications from Whole Cell Extracts in Saccharomyces cerevisiae Lindsay G. Ball and Wei Xiao20. Ligand Engineering Using Yeast Surface Display Benjamin J. Hackel21. Screening Combinatorial Libraries of Cyclic Peptides Using the Yeast Two-Hybrid Assay Kris Barreto and C. Ronald Geyer

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