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Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls

Table of Contents

Live imaging of the cytoskeleton in early cleavage stage zebrafish embryos.Martin Wühr, Nikolaus D. Obholzer, Sean G. Megason, H. William Detrich, III, Timothy J. Mitchison
Analysis of Cell Proliferation, Senescence and Cell Death in Zebrafish EmbryosDaniel Verduzco and James F. Amatruda
Analysis of Cilia Structure and Function in Zebrafish Jarema Malicki, Andrei Avanesov, Jade Li, Shiaulou Yuan, Zhaoxia Sun
Cellular Dissection of Zebrafish HematopoiesisDavid L Stachura, David Traver
Zebrafish lipid metabolism: From mediating early patterning to the metabolism of dietary fat and cholesterolJennifer L. Anderson, Juliana D. Carten, Steven A. Farber
Part II: Developmental and Neural Biology

Development of the Zebrafish Enteric Nervous SystemIain Shepherd, Judith Eisen
A Guide to Analysis of Cardiac Phenotypes in the Zebrafish EmbryoDeborah Yelon, Grant I. Miura
Chemical Approaches to Angiogenesis in Development and RegenerationJoanne Chan, Sean Hasso
Laser-induced Thrombosis in ZebrafishPudur Jagadeeswaran, Maira Carrillo, Uvaraj P. Radhakrishnan, Surendra K. Rajpurohit and Seongcheol Kim
Endoderm Specification, Liver Development, and RegenerationTrista E. North, Wolfram Goessling
Morphogenesis of the zebrafish jaw: Development beyond the embryoKevin J. Parsons, Viktoria Andreeva, W. James Cooper, Pamela C. Yelick, R. Craig Albertson
Associative learning in zebrafish (Danio rerio)Robert Gerlai

About the Author

Professor of Biochemistry and Marine Biology at Northeastern University, promoted 1996. Joined Northeastern faculty in 1987. Previously a faculty member in Dept. of Biochemistry at the University of Mississippi Medical Center, 1983-1987.Principal Investigator in the U.S. Antarctic Program since 1984. Twelve field seasons "on the ice" since 1981. Research conducted at Palmer Station, Antarctica, and McMurdo Station, Antarctica.Research areas: Biochemical, cellular, and physiological adaptation to low and high temperatures. Structure and function of cytoplasmic microtubules and microtubule-dependent motors from cold-adapted Antarctic fishes. Regulation of tubulin and globin gene expression in zebrafish and Antarctic fishes. Role of microtubules in morphogenesis of the zebrafish embryo. Developmental hemapoiesis in zebrafish and Antarctic fishes. UV-induced DNA damage and repair in Antarctic marine organisms.